Denniston M L, Uyeki E M
J Toxicol Environ Health. 1987;21(3):375-86. doi: 10.1080/15287398709531026.
Chinese hamster ovary cells were cultured with chromium-51 chromate to study the site of chromium interaction with cell biomolecules. After incubation, cells were homogenized and separated into nuclear, mitochondrial, microsomal, and cytosolic fractions. Greater than 75% of the radioactivity was found in the cytosolic fraction. The supernatant from the centrifuged cell homogenate, which contained greater than 90% of the chromium radioactivity, was subjected to chromatographic investigation. The combination of anion exchange and ion-pair high-performance liquid chromatography (HPLC) showed that at least three different molecular species interact with chromate or its reduced derivative, Cr(III). These species are glutathione, the nucleotides cytosine triphosphate, uridine triphosphate, guanine triphosphate, adenosine triphosphate, and adenosine diphosphate, plus an as yet unknown species of protein or peptide. Preliminary data for the specific activity of nucleoside triphosphates range from 6000 to 18,000 cpm/micrograms ribonucleoside triphosphate. The glutathione accounted for 50% of the observed radioactivity, the nucleotides for 30%, and the metalloprotein accounted for the remainder.
用铬-51铬酸盐培养中国仓鼠卵巢细胞，以研究铬与细胞生物分子相互作用的位点。孵育后，将细胞匀浆并分离成细胞核、线粒体、微粒体和胞质部分。超过75%的放射性存在于胞质部分。离心后的细胞匀浆上清液含有超过90%的铬放射性，对其进行了色谱研究。阴离子交换和离子对高效液相色谱（HPLC）的结合表明，至少有三种不同的分子物种与铬酸盐或其还原衍生物Cr(III)相互作用。这些物种是谷胱甘肽、核苷酸三磷酸胞嘧啶、三磷酸尿苷、三磷酸鸟苷、三磷酸腺苷和二磷酸腺苷，外加一种尚未确定的蛋白质或肽物种。三磷酸核苷的比活性初步数据范围为6000至18000 cpm/微克核糖核苷三磷酸。谷胱甘肽占观察到的放射性的50%，核苷酸占30%，金属蛋白占其余部分。
