Woodward W R, Seil F J, Hammerstad J P
J Neurosci Res. 1987;17(2):184-8. doi: 10.1002/jnr.490170214.
In contrast to locus coeruleus neurons in vivo, dopamine was the predominant catecholamine synthesized, stored, and released by neonatal mouse locus coeruleus cultures which included target cerebellar tissue, and norepinephrine was present in these cultures only at very low levels. Developmentally, norepinephrine increased slightly in the explants during the first 4 days in vitro and declined thereafter to barely detectable levels, whereas dopamine began to rise after 4 days and reached maximal levels by 7 days. Dopamine beta-hydroxylase was present in these cultures throughout maturation. These results suggest that the high ratio of dopamine to norepinephrine in locus coeruleus cultures cannot be attributed to the absence of appropriate target tissue or to a lack of the enzyme, dopamine beta-hydroxylase.
与体内的蓝斑神经元不同，多巴胺是由包含靶小脑组织的新生小鼠蓝斑培养物合成、储存和释放的主要儿茶酚胺，而去甲肾上腺素在这些培养物中的含量极低。在发育过程中，去甲肾上腺素在体外培养的前4天略有增加，此后下降至几乎检测不到的水平，而多巴胺在4天后开始上升，并在7天时达到最高水平。在整个成熟过程中，多巴胺β-羟化酶都存在于这些培养物中。这些结果表明，蓝斑培养物中多巴胺与去甲肾上腺素的高比例不能归因于缺乏合适的靶组织或缺乏多巴胺β-羟化酶。
