National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, China.
Cell Reprogram. 2022 Aug;24(4):175-185. doi: 10.1089/cell.2022.0015. Epub 2022 Jul 20.
The oocyte maturation (IVM) technique is important in animal husbandry, biomedicine, and human-assisted reproduction. However, the developmental potential of matured oocytes is usually lower than that of matured (IVVM) oocytes. Amphiregulin (AREG) is an EGF-like growth factor that plays critical roles in the maturation and development of mammalian oocytes. This study investigated the effects of AREG supplementation during pig oocyte IVM on the subsequent development of cloned embryos. The addition of AREG to pig oocyte IVM medium improved the developmental competence of treated oocyte-derived cloned embryos by enhancing the expansion and proliferation of cumulus cells (CCs) during IVM. The positive effect of AREG on enhancing the quality of IVVM pig oocytes might be due to the activation of proliferation-related pathways in CCs by acting on the AREG receptor. The present study provides an AREG treatment-based method to improve the developmental competence of cloned pig embryos.
卵母细胞成熟（IVM）技术在畜牧业、生物医学和人类辅助生殖中具有重要意义。然而，成熟卵母细胞的发育潜能通常低于体外成熟（IVVM）卵母细胞。 Amphiregulin (AREG) 是一种 EGF 样生长因子，在哺乳动物卵母细胞的成熟和发育中发挥关键作用。本研究探讨了在猪卵母细胞 IVM 期间添加 AREG 对随后克隆胚胎发育的影响。在 IVM 期间，向猪卵母细胞 IVM 培养基中添加 AREG 可通过增强卵丘细胞（CCs）的扩张和增殖，提高处理卵母细胞衍生的克隆胚胎的发育能力。AREG 通过作用于 AREG 受体激活 CCs 中的增殖相关途径，从而增强 IVVM 猪卵母细胞质量的积极作用。本研究提供了一种基于 AREG 处理的方法来提高克隆猪胚胎的发育能力。
