State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science, Guangxi Normal University, Guilin 541004, PR China; College of Food and Bioengineering, Hezhou University, Hezhou 542899, PR China.
College of Food and Bioengineering, Hezhou University, Hezhou 542899, PR China.
J Colloid Interface Sci. 2022 Dec;627:405-414. doi: 10.1016/j.jcis.2022.07.036. Epub 2022 Jul 13.
Nanozymes-functional materials that possess intrinsic enzyme-like characteristics-have gained tremendous attention in recent years owing to their unique advantages; however, further research is required to understand their scope in biological applications. In this study, dextran-coated nanogadolinia (DCNG) was synthesised, and its phosphatase mimetic activity was demonstrated. Specifically, the dephosphorylation of adenosine triphosphate (ATP), an important biomolecule, by DCNG was investigated. The results showed that DCNG could selectively catalyse the hydrolysis of the terminal high-energy phosphate bonds of ATP under physiological conditions. Furthermore, the biocompatible DCNG, with remarkable phosphatase mimicking activity, decreased the intracellular ATP content by dephosphorylation and increased ethanol yield during glucose fermentation by S. cerevisiae. These results indicate potential alternatives for improving ethanol yields and exploring novel biological applications of nanozymes.
纳米酶——具有固有酶样特性的功能材料——近年来因其独特的优势而引起了极大的关注；然而，需要进一步的研究来了解它们在生物应用中的范围。在这项研究中，合成了葡聚糖包覆的纳米钆（DCNG），并证明了其磷酸酶模拟活性。具体来说，研究了 DCNG 对重要生物分子三磷酸腺苷（ATP）的去磷酸化作用。结果表明，DCNG 可以在生理条件下选择性地催化 ATP 末端高能磷酸键的水解。此外，具有显著磷酸酶模拟活性的生物相容性 DCNG 通过去磷酸化作用降低了细胞内 ATP 的含量，并提高了酿酒酵母葡萄糖发酵过程中的乙醇产量。这些结果表明，纳米酶在提高乙醇产量和探索新的生物应用方面具有潜在的替代方法。
