State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China; Precision Livestock and Nutrition Unit, Gembloux Agro-Bio Tech, TERRA Teaching and Research Centre, Liège University, Gembloux, Belgium.
J Nutr Biochem. 2022 Nov;109:109107. doi: 10.1016/j.jnutbio.2022.109107. Epub 2022 Jul 19.
During weaning, infants and young animals are susceptible to severe enteric infections, thus inducing intestinal microbiota dysbiosis, intestinal inflammation, and impaired intestinal barrier function. Pectin (PEC), a prebiotic polysaccharide, enhances intestinal health with the potential for a therapeutic effect on intestinal diseases. One 21-d study was conducted to investigate the protective effect of pectin against intestinal injury induced by intraperitoneal injection of Escherichia coli lipopolysaccharide (LPS) in a piglet model. A total of 24 piglets (6.77±0.92 kg BW; Duroc × Landrace × Large White; barrows; 21 d of age) were randomly assigned into three groups: control group, LPS-challenged group, and PEC + LPS group. Piglets were administrated with LPS or saline on d14 and d21 of the experiment. All piglets were slaughtered and intestinal samples were collected after 3 h administration on d21. Pectin supplementation ameliorated the LPS-induced inflammation response and damage to the ileal morphology. Meanwhile, pectin also improved intestinal mucin barrier function, increased the mRNA expression of MUC2, and improved intestinal mucus glycosylation. LPS challenge reduced the diversity of intestinal microbiota and enriched the relative abundance of Helicobacter. Pectin restored alpha diversity and improved the structure of the gut microbiota by enriching anti-inflammatory bacteria and short-chain fatty acids (SCFAs)-producing bacteria, and increased the concentrations of acetate. In addition, Spearman rank correlation analysis also revealed the potential relationship between intestinal microbiota and intestinal morphology, intestinal inflammation, and intestinal glycosylation in piglets. Taken together, these results indicate that pectin enhances intestinal integrity and barrier function by altering intestinal microbiota composition and their metabolites, which subsequently alleviates intestinal injury and finally improves the growth performance of piglets.
在断奶期间，婴儿和幼小动物易患严重的肠道感染，从而导致肠道微生物群落失调、肠道炎症和肠道屏障功能受损。果胶（PEC）是一种益生元多糖，可增强肠道健康，并有治疗肠道疾病的潜力。本研究进行了一项为期 21 天的研究，以调查果胶对仔猪模型中腹腔注射大肠杆菌脂多糖（LPS）引起的肠道损伤的保护作用。将 24 头仔猪（6.77±0.92 kg BW；杜洛克×长白×大白；公猪；21 日龄）随机分为 3 组：对照组、LPS 挑战组和 PEC+LPS 组。仔猪在实验的第 14 天和第 21 天接受 LPS 或生理盐水处理。所有仔猪在第 21 天给药后 3 h 屠宰并采集肠道样本。果胶补充剂改善了 LPS 诱导的炎症反应和回肠形态损伤。同时，果胶还改善了肠道粘蛋白屏障功能，增加了 MUC2 的 mRNA 表达，并改善了肠道粘液糖基化。LPS 挑战降低了肠道微生物群的多样性并富集了相对丰度较高的幽门螺旋杆菌。果胶通过富集抗炎细菌和产生短链脂肪酸（SCFAs）的细菌来恢复 alpha 多样性并改善肠道微生物群的结构，并增加了乙酸盐的浓度。此外，Spearman 等级相关分析还揭示了仔猪肠道微生物群与肠道形态、肠道炎症和肠道糖基化之间的潜在关系。总之，这些结果表明，果胶通过改变肠道微生物群落组成及其代谢物来增强肠道完整性和屏障功能，从而减轻肠道损伤，最终提高仔猪的生长性能。
