Wu Yuliang, Li Yuxin, Chen Mengli, Zhao Juan, Xiong Xia, Olnood Chen Guang, Gao Yundi, Wang Fei, Peng Can, Liu Miao, Huang Chunxia, Li Jianzhong, He Liuqin, Yang Huansheng, Yin Yulong
Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, China.
CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.
Front Vet Sci. 2025 Mar 10;12:1533872. doi: 10.3389/fvets.2025.1533872. eCollection 2025.
The intestine is the largest immune and barrier organ in the body, and diarrhea and even death during piglet development are related to dysfunction caused by intestinal barrier damage and inflammation. A water-soluble β-glucan produced by Agrobacterium ZX09 has been shown to have a beneficial effect on gastrointestinal health. The main objective of this study was to investigate whether pre-feeding β-glucan has a protective effect on LPS-induced immune stress in piglets. In this study, 24 weaned piglets (21-day-old; 6.64 ± 0.16 kg) were assigned to 4 treatments in a two × two factorial design with diet (with or without β-glucan) and immunological challenge (saline or LPS). Piglets were challenged with saline or LPS after 39 days of feeding 0 or 200 mg/kg β-glucan. The results demonstrated that β-glucan supplementation increased the average daily weight gain and daily feed intake, and decreased diarrhea rate of piglets. Intestinal inflammation symptoms and histological changes in LPS-challenged piglets were alleviated by pre-feeding of β-glucan. β-glucan supplementation reduced serum IL-1β (interleukin-1β) and NO (nitric oxide) secretion in piglets after LPS challenge (0.01 < < 0.05). Supplementation with β-glucan downregulated the mRNA expression of IL-6 in piglets after LPS challenge (0.01 < < 0.05). β-glucan supplementation enriched the short-chain fatty acid-producing bacteria, such as and (0.01 < < 0.05), and increased the concentrations of propionate and butyrate (0.01 < < 0.05). In conclusion, pre-feeding β-glucan can enhance piglet immunity and promote piglet growth by influencing gut microbiota composition and metabolism, and alleviate intestinal damage after LPS challenge.
肠道是机体最大的免疫和屏障器官，仔猪发育过程中的腹泻甚至死亡与肠道屏障损伤和炎症引起的功能障碍有关。农杆菌ZX09产生的一种水溶性β-葡聚糖已被证明对胃肠道健康有益。本研究的主要目的是探讨预饲β-葡聚糖对仔猪脂多糖（LPS）诱导的免疫应激是否具有保护作用。在本研究中，24头断奶仔猪（21日龄；体重6.64±0.16 kg）采用二×二析因设计，分为4组，分别给予含或不含β-葡聚糖的日粮，并进行免疫挑战（生理盐水或LPS）。在饲喂0或200 mg/kg β-葡聚糖39天后，仔猪接受生理盐水或LPS攻击。结果表明，补充β-葡聚糖可提高仔猪的平均日增重和日采食量，并降低腹泻率。预饲β-葡聚糖可减轻LPS攻击仔猪的肠道炎症症状和组织学变化。补充β-葡聚糖可降低LPS攻击后仔猪血清白细胞介素-1β（IL-1β）和一氧化氮（NO）的分泌（0.01< <0.05）。补充β-葡聚糖可下调LPS攻击后仔猪IL-6的mRNA表达（0.01< <0.05）。补充β-葡聚糖可富集产生短链脂肪酸的细菌，如 和 （0.01< <0.05），并增加丙酸和丁酸的浓度（0.01< <0.05）。总之，预饲β-葡聚糖可通过影响肠道微生物群组成和代谢来增强仔猪免疫力、促进仔猪生长，并减轻LPS攻击后的肠道损伤。
