State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. China; College of Animal Science and Technology, Hunan Agricultural University, Changsha, P. R. China.
J Nutr Biochem. 2024 Feb;124:109491. doi: 10.1016/j.jnutbio.2023.109491. Epub 2023 Oct 19.
Weaning is one of the major factors that cause stress and intestinal infection in infants and in young animals due to an immature intestine and not fully developed immune functions. Pectin (PEC), a prebiotic polysaccharide, has attracted considerable attention in intestinal epithelial signaling and function via modulation of the microbial community. A total of 16 weaned piglets (21-d-old) were randomly assigned into two groups: control group and PEC group. Supplementation of 5% pectin improved intestinal mucosal barrier function by modulating the composition of the bile acid pool in piglets. Specifically, piglets in PEC group had less serum D-lactate content and alkaline phosphatase activity. In the ileum, dietary pectin increased the number of crypt PAS/AB-positive goblet cells and the mRNA expressions of MUC2, ZO-1, and Occludin. Piglets in PEC group displayed a decreased abundance of Enterococcus (2.71 vs. 65.92%), but the abundances of Lactobacillus (30.80 vs. 7.93%), Streptococcus (21.41 vs. 14.81%), and Clostridium_sensu_stricto_1 (28.34 vs. 0.01%) were increased. Elevated concentrations of bile acids especially hyocholic acid species (HCAs) including HCA, HDCA, and THDCA were also observed. Besides, correlation analysis revealed that dietary pectin supplementation may have beneficial effects through stimulation of the crosstalk between gut microbes and bile acid synthesis within the enterohepatic circulation. Thus, dietary pectin supplementation exhibited a further positive effect on the healthy growth and development of weaned piglets. These findings suggest pectin supplementation as the prebiotic is beneficial for gut health and improvement of weaned stress via regulating microbiota and bile acid metabolism.
断奶是导致婴儿和幼小动物出现应激和肠道感染的主要因素之一，这是由于它们的肠道不成熟且免疫功能尚未完全发育。果胶（PEC）作为一种益生元多糖，通过调节微生物群落，在肠道上皮细胞信号转导和功能方面引起了相当大的关注。将 16 头 21 日龄断奶仔猪随机分为两组：对照组和 PEC 组。补充 5%的果胶通过调节胆汁酸库的组成改善了仔猪的肠道黏膜屏障功能。具体来说，PEC 组仔猪的血清 D-乳酸含量和碱性磷酸酶活性较低。在回肠中，日粮果胶增加了隐窝 PAS/AB 阳性杯状细胞的数量以及 MUC2、ZO-1 和 Occludin 的 mRNA 表达。PEC 组仔猪肠球菌（2.71%比 65.92%）丰度降低，但乳杆菌（30.80%比 7.93%）、链球菌（21.41%比 14.81%）和梭菌属 sensu stricto_1（28.34%比 0.01%）丰度增加。胆汁酸，特别是胆酸（HCAs）包括 HCA、HDCA 和 THDCA 的浓度也升高。此外，相关性分析表明，日粮果胶的补充可能通过刺激肠道微生物与肠肝循环中胆汁酸合成之间的串扰产生有益效果。因此，日粮果胶的补充对断奶仔猪的健康生长和发育具有进一步的积极影响。这些发现表明，果胶作为一种益生元，通过调节微生物群和胆汁酸代谢，有利于肠道健康和改善断奶应激。
