Department of Chemistry and Biochemistry, Faculty of Arts and Science, Concordia University, 7141 Rue Sherbrooke Ouest, Montréal, Québec H4B 1R6, Canada.
Org Lett. 2022 Aug 5;24(30):5579-5583. doi: 10.1021/acs.orglett.2c02149. Epub 2022 Jul 21.
The synthesis and incorporation of a tosylated phosphoramidite linker containing a disulfide bond is described. Incorporation of the linker into short DNA and RNA oligomers proceeded efficiently using automated solid phase synthesis. Treatment of the support bound oligonucleotide followed by cleavage from the solid support provided a variety of common functional handles, expanding the strategies of bifunctional modification of synthetic oligonucleotides for conjugation applications.
描述了一种含有二硫键的 tosylated 磷酰胺酯连接子的合成和掺入。该连接子可通过自动化固相合成有效地掺入短 DNA 和 RNA 寡核苷酸中。随后对连接在载体上的寡核苷酸进行处理，并从固体载体上切割下来，提供了各种常见的功能接头，扩展了用于连接应用的合成寡核苷酸双官能修饰的策略。
