一种改良的肾保留式门静脉动脉化同种异位辅助性肝移植模型可增加肝脏中 IL-6、TNF-α 和 HGF 的水平，增强肝脏再生：动物模型。

A modified kidney-sparing portal vein arterialization model of heterotopic auxiliary liver transplantation increases liver IL-6, TNF-α, and HGF levels and enhances liver regeneration: an animal model.

机构信息

Department of Hepatobiliary, Pancreatic and Splenic Surgery, The Affiliated Hospital of Inner Mongolia Medical University, Huhhot, 010050, People's Republic of China.

Department of General Surgery, Hohhot First Hospital, Huhhot, 010030, People's Republic of China.

出版信息

BMC Surg. 2022 Jul 21;22(1):281. doi: 10.1186/s12893-022-01726-5.

DOI:10.1186/s12893-022-01726-5

PMID:35864504

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9306035/

Abstract

BACKGROUND AND AIM

The success of partial donor liver transplantation is affected by the implantation site of the donor liver and the vascular reconstruction approach. We investigated the effects of different donor liver implantation sites and vascular reconstruction approaches on liver regeneration using a rat kidney-sparing heterotopic auxiliary liver transplantation model, with portal vein arterialization (PVA).

METHODS

Sixty male Sprague-Dawley rats underwent end-to-end anastomosis of the donor liver portal vein and the right renal artery stent (control group), or end-to-side anastomosis of the donor liver portal vein and the left common iliac artery (experimental group).

RESULTS

The experimental group had significantly lower plasma levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin, and cholinesterase than the control group (all, P < 0.05). The levels of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and hepatocyte growth factor (HGF) in the liver were significantly higher in the experimental group than that in the control group (all, P < 0.05). Hematoxylin and eosin (HE) staining of the liver tissue specimens indicated that the experimental group had greater hepatocyte regeneration compared to the control group.

CONCLUSIONS

The modified kidney-sparing PVA model of heterotopic auxiliary liver transplantation is more conducive to liver regeneration with quicker return of liver function.

摘要

背景与目的

部分供肝移植的成功受供肝植入部位和血管重建方法的影响。我们使用大鼠肾保护异位辅助性肝移植模型（门静脉动脉化）研究了不同供肝植入部位和血管重建方法对肝再生的影响。

方法

60 只雄性 Sprague-Dawley 大鼠行供肝门静脉与右肾动脉支架端端吻合（对照组）或供肝门静脉与左髂总动脉端侧吻合（实验组）。

结果

实验组的血浆丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总胆红素和胆碱酯酶水平明显低于对照组（均 P<0.05）。实验组肝组织肿瘤坏死因子-α（TNF-α）、白细胞介素 6（IL-6）和肝细胞生长因子（HGF）水平明显高于对照组（均 P<0.05）。肝组织标本苏木精和伊红（HE）染色显示实验组较对照组有更大的肝细胞再生。