Department of Hepatobiliary, Pancreatic and Splenic Surgery, The Affiliated Hospital of Inner Mongolia Medical University, Huhhot, 010050, People's Republic of China.
Department of General Surgery, Hohhot First Hospital, Huhhot, 010030, People's Republic of China.
BMC Surg. 2022 Jul 21;22(1):281. doi: 10.1186/s12893-022-01726-5.
The success of partial donor liver transplantation is affected by the implantation site of the donor liver and the vascular reconstruction approach. We investigated the effects of different donor liver implantation sites and vascular reconstruction approaches on liver regeneration using a rat kidney-sparing heterotopic auxiliary liver transplantation model, with portal vein arterialization (PVA).
Sixty male Sprague-Dawley rats underwent end-to-end anastomosis of the donor liver portal vein and the right renal artery stent (control group), or end-to-side anastomosis of the donor liver portal vein and the left common iliac artery (experimental group).
The experimental group had significantly lower plasma levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin, and cholinesterase than the control group (all, P < 0.05). The levels of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and hepatocyte growth factor (HGF) in the liver were significantly higher in the experimental group than that in the control group (all, P < 0.05). Hematoxylin and eosin (HE) staining of the liver tissue specimens indicated that the experimental group had greater hepatocyte regeneration compared to the control group.
The modified kidney-sparing PVA model of heterotopic auxiliary liver transplantation is more conducive to liver regeneration with quicker return of liver function.
部分供肝移植的成功受供肝植入部位和血管重建方法的影响。我们使用大鼠肾保护异位辅助性肝移植模型（门静脉动脉化）研究了不同供肝植入部位和血管重建方法对肝再生的影响。
60 只雄性 Sprague-Dawley 大鼠行供肝门静脉与右肾动脉支架端端吻合（对照组）或供肝门静脉与左髂总动脉端侧吻合（实验组）。
实验组的血浆丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总胆红素和胆碱酯酶水平明显低于对照组（均 P<0.05）。实验组肝组织肿瘤坏死因子-α（TNF-α）、白细胞介素 6（IL-6）和肝细胞生长因子（HGF）水平明显高于对照组（均 P<0.05）。肝组织标本苏木精和伊红（HE）染色显示实验组较对照组有更大的肝细胞再生。
改良的肾保护型异位辅助性肝移植门静脉动脉化模型更有利于肝再生，肝功能恢复更快。
