Li Jun, Zhang Yujun, Ren Jianjun, Zhang Junjing, Qiao Jianliang, Meng Xingkai
Department of General Surgery, Xuanwu Hospital, Capital Medical UniversityBeijing 100053, P. R. China; Department of General Surgery, The Affiliated Hospital of Inner Mongolia Medical UniversityHohhot 010050, Inner Mongolia Autonomous Region, P. R. China.
Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University Chongqing 400038, P. R. China.
Int J Clin Exp Med. 2015 Oct 15;8(10):18638-46. eCollection 2015.
To establish an animal model of improved donor liver position selection and revascularization for heterotopic auxiliary liver transplantation with portal vein arterialization (HALT-PVA).
Sprague-Dawley rats were utilized to establish models. Improved HALT-PVA was conducted for the experimental rat: hepatic common artery of donor liver was end-to-side anastomosed to portal vein which was end-to-side anastomosed to the left common iliac artery of host rat, while the segments of inferior vena cava superior and inferior to the donor liver were end-to-side anastomosed to the inferior vena cava of host rat, respectively. For the control rats, liver transplantations were conducted through end-to-end anastomosis between portal vein of donor liver and stand tube placed in right renal artery of host rat, and end-to-side anastomosis between the inferior vena cava inferior to the donor liver with the inferior vena cava of host rat, while the inferior vena cava superior to the donor liver was stitched up. Besides, hepaticoenterostomy were performed to all rats and survival status were monitored. ALT, AST, TBil and CHE were tested continuously after operation, and pathological examination of liver tissues were performed.
The survival rate was 93.3% (14/15). ALT, AST, TBil and CHE for experimental group showed a rapider recovery of liver functions than controls. Pathological examinations of liver tissues from the experimental-group rats showed better presentation than the control-group rats.
The improved HALT-PVA better accords with the normal anatomy, with little detriment to implanted liver, and therefore is a good model for HALT-PVA related research.
建立一种用于门静脉动脉化的异位辅助肝移植（HALT-PVA）中改善供肝位置选择和血管重建的动物模型。
使用Sprague-Dawley大鼠建立模型。对实验大鼠进行改良的HALT-PVA：将供肝的肝总动脉与门静脉进行端侧吻合，门静脉再与宿主大鼠的左髂总动脉进行端侧吻合，同时将供肝上下方的下腔静脉段分别与宿主大鼠的下腔静脉进行端侧吻合。对于对照大鼠，通过将供肝门静脉与置于宿主大鼠右肾动脉的套管进行端端吻合，以及将供肝下方的下腔静脉与宿主大鼠的下腔静脉进行端侧吻合来进行肝移植，而将供肝上方的下腔静脉缝合。此外，对所有大鼠进行肝肠吻合术并监测生存状态。术后连续检测谷丙转氨酶（ALT）、谷草转氨酶（AST）、总胆红素（TBil）和胆碱酯酶（CHE），并对肝组织进行病理检查。
存活率为93.3%（14/15）。实验组的ALT、AST、TBil和CHE显示肝功能恢复比对照组更快。实验组大鼠肝组织的病理检查显示比对照组更好的表现。
改良的HALT-PVA更符合正常解剖结构，对植入肝脏的损害较小，因此是HALT-PVA相关研究的良好模型。
