用（膜翅目：蜜蜂科）启动和监测大黄蜂微型蜂群的方案。
Protocol for Initiating and Monitoring Bumble Bee Microcolonies with (Hymenoptera: Apidae).
作者信息
Lehmann David M
机构信息
Center for Public Health and Environmental Assessment (CPHEA), Health and Environmental Effects Assessment Division, Integrated Health Assessment Branch, U.S. - Environmental Protection Agency, Research Triangle Park, NC, 27711, USA.
出版信息
Bio Protoc. 2022 Jun 20;12(12). doi: 10.21769/BioProtoc.4451.
Populations of some bumble bee species are in decline, prompting the need to better understand bumble bee biology and for assessing the effects of environmental stressors on these important pollinators. Microcolonies have been successfully used for investigating a range of endpoints, including behavior, gut microbiome, nutrition, development, pathogens, and the effects of pesticide exposure on bumble bee health. Here, we present a step-by-step protocol for initiating, maintaining, and monitoring microcolonies with . This protocol has been successfully used in two pesticide exposure-effects studies and can be easily expanded to investigate other aspects of bumble bee biology.
一些熊蜂物种的数量正在减少，这促使人们需要更好地了解熊蜂生物学，并评估环境压力源对这些重要传粉者的影响。微型蜂群已成功用于研究一系列指标，包括行为、肠道微生物群、营养、发育、病原体以及农药暴露对熊蜂健康的影响。在此，我们提供了一个使用[具体内容未给出]启动、维持和监测微型蜂群的详细方案。该方案已成功应用于两项农药暴露效应研究，并且可以轻松扩展以研究熊蜂生物学的其他方面。
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