Determination of aflatoxin B1 in based on aptamer magnetic solid-phase extraction.

Aflatoxin B1 (AFB) is considered as the most prevalent and toxic mycotoxin in food, and is the indispensable index in the monitoring of , a traditional chinese fermented bean paste from Sichuan. However, the effeciency of AFB detection in is influenced by the traditional extraction, which is usually complex and time consuming. Therefore, an aptamer-based magnetic solid-phase extraction method was designed for the pretreatment of AFB in this sample, for which FeO was synthesized the solvothermal method and then a FeO@SiO-NH with a core-shell structure was prepared, followed by an AFB-aptamer attachment. The validation was performed an enzyme-linked immunosorbent assay and compared with HPLC-MS/MS. The linearity range of this method was 0.5-2.0 ng mL with of 0.981, and recoveries of AFB ranged from 80.19% to 113.92% with RSDs below 7.28% with no significant differences compared to HPLC-MS/MS. The three-time reusability efficiencies of aptamer-MNPs were averaged at 78.24%. The results proved that aptamer-MNPs were high-performance adsorbents for extracting and enriching AFB, facilitating quick and effective detection of AFB in samples.