Zeng Chaoyi, Xu Chi, Tian Hongyun, Shao Kun, Song Yaning, Yang Xiao, Che Zhenming, Huang Yukun
School of Food and Biological Engineering, Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Xihua University Chengdu 610039 China
Department of Food Biotechnology, Faculty of Biotechnology, Assumption University Bangkok 10240 Thailand.
RSC Adv. 2022 Jul 6;12(30):19528-19536. doi: 10.1039/d2ra02763a. eCollection 2022 Jun 29.
Aflatoxin B1 (AFB) is considered as the most prevalent and toxic mycotoxin in food, and is the indispensable index in the monitoring of , a traditional chinese fermented bean paste from Sichuan. However, the effeciency of AFB detection in is influenced by the traditional extraction, which is usually complex and time consuming. Therefore, an aptamer-based magnetic solid-phase extraction method was designed for the pretreatment of AFB in this sample, for which FeO was synthesized the solvothermal method and then a FeO@SiO-NH with a core-shell structure was prepared, followed by an AFB-aptamer attachment. The validation was performed an enzyme-linked immunosorbent assay and compared with HPLC-MS/MS. The linearity range of this method was 0.5-2.0 ng mL with of 0.981, and recoveries of AFB ranged from 80.19% to 113.92% with RSDs below 7.28% with no significant differences compared to HPLC-MS/MS. The three-time reusability efficiencies of aptamer-MNPs were averaged at 78.24%. The results proved that aptamer-MNPs were high-performance adsorbents for extracting and enriching AFB, facilitating quick and effective detection of AFB in samples.
黄曲霉毒素B1（AFB）被认为是食品中最普遍且毒性最强的霉菌毒素，是监测四川传统发酵豆瓣酱的一项不可或缺的指标。然而，豆瓣酱中AFB的检测效率受到传统提取方法的影响，传统方法通常复杂且耗时。因此，本文设计了一种基于适配体的磁性固相萃取方法用于该样品中AFB的预处理，通过溶剂热法合成FeO，然后制备具有核壳结构的FeO@SiO-NH，随后连接AFB适配体。通过酶联免疫吸附测定法进行验证，并与HPLC-MS/MS进行比较。该方法的线性范围为0.5 - 2.0 ng mL，相关系数为0.981，AFB的回收率在80.19%至113.92%之间，相对标准偏差低于7.28%，与HPLC-MS/MS相比无显著差异。适配体修饰的磁性纳米颗粒的三次重复使用效率平均为78.24%。结果证明，适配体修饰的磁性纳米颗粒是用于提取和富集AFB的高性能吸附剂，有助于快速有效地检测豆瓣酱样品中的AFB。
