Soerjawinata Winda, Kockler Isabelle, Wommer Lars, Frank Robert, Schüffler Anja, Schirmeister Tanja, Ulber Roland, Kampeis Percy
Institute for Biotechnical Process Design Trier University of Applied Sciences, Environmental Campus Birkenfeld Hoppstädten-Weiersbach Germany.
Institut für Biotechnologie und Wirkstoff-Forschung gGmbH (IBWF) Mainz Germany.
Eng Life Sci. 2022 May 18;22(7):474-483. doi: 10.1002/elsc.202100094. eCollection 2022 Jul.
This study introduced an automated long-term fermentation process for fungals grown in pellet form. The goal was to reduce the overgrowth of bioreactor internals and sensors while better rheological properties in the fermentation broth, such as oxygen transfer and mixing time, can be achieved. Because this could not be accomplished with continuous culture and fed-batch fermentation, repeated-batch fermentation was implemented with the help of additional bioreactor internals ("sporulation supports"). This should capture some biomass during fermentation. After harvesting the suspended biomass, intermediate cleaning was performed using a cleaning device. The biomass retained on the sporulation support went through the sporulation phase. The spores were subsequently used as inocula for the next batch. The reason for this approach was that the retained pellets could otherwise cause problems (., overgrowth on sensors) in subsequent batches because the fungus would then show undesirable hyphal growth. Various sporulation supports were tested for sufficient biomass fixation to start the next batch. A reproducible spore concentration within the range of the requirements could be achieved by adjusting the sporulation support (design and construction material), and an intermediate cleaning adapted to this.
本研究介绍了一种用于以颗粒形式生长的真菌的自动化长期发酵工艺。目标是减少生物反应器内部构件和传感器的过度生长，同时在发酵液中实现更好的流变学特性，如氧传递和混合时间。由于连续培养和补料分批发酵无法实现这一目标，因此在额外的生物反应器内部构件（“孢子形成载体”）的帮助下实施了重复分批发酵。这应该在发酵过程中捕获一些生物质。收获悬浮生物质后，使用清洁装置进行中间清洁。保留在孢子形成载体上的生物质进入孢子形成阶段。随后，孢子被用作下一批的接种物。采用这种方法的原因是，否则保留的颗粒可能会在后续批次中导致问题（如传感器上的过度生长），因为真菌随后会出现不良的菌丝生长。测试了各种孢子形成载体，以确保有足够的生物质固定来开始下一批发酵。通过调整孢子形成载体（设计和建筑材料）以及与之相适应的中间清洁，可以实现要求范围内可重复的孢子浓度。
