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用[具体菌种名称]生产蛋白酶抑制剂——颗粒形式生长培养基的优化及细胞毒性测试

Production of Protease Inhibitor With sp. - Optimization of the Medium for Growth in Pellet Form and Cytotoxicity Testing.

作者信息

Soerjawinata Winda, Prajapati Shila, Barth Isabelle, Lu Xiaohua, Ulber Roland, Efferth Thomas, Kampeis Percy

机构信息

Environmental Campus Birkenfeld Institute for Biotechnical Process Design Trier University of Applied Sciences Birkenfeld Germany.

Institute of Pharmaceutical and Biomedical Sciences Johannes Gutenberg University Mainz Mainz Germany.

出版信息

Eng Life Sci. 2025 Mar 17;25(3):e70012. doi: 10.1002/elsc.70012. eCollection 2025 Mar.

DOI:10.1002/elsc.70012
PMID:40104836
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11913720/
Abstract

sp. (IBWF 040-09) produces a protease inhibitor that can potentially be used against the main protease of human African trypanosomiasis. Since the target substance is formed intracellularly (under nutrient limitation), the fungal pellet is preferred compared to the free mycelia in bioreactor cultivation. The optimization of the production of protease inhibitor became the main focus of this study. The effects of the concentrations of spores, calcium chloride, and Pluronic F68 were investigated with regard to fungal growth, pellet morphology, and the production of protease inhibitor. The combination of adjusting the spore concentration and adding Pluronic F68 and calcium chloride increased the probability of achieving the desired morphology. This ensured better reproducibility of the production of the target substance by sp. (IBWF 040-09) with the bioreactor system used. In addition, the protease inhibitor was tested in a resazurin assay and showed no noticeable cytotoxic effects on peripheral blood mononuclear cells isolated from whole blood cells.

摘要

菌株（编号为IBWF 040 - 09）产生一种蛋白酶抑制剂，该抑制剂有可能用于对抗人类非洲锥虫病的主要蛋白酶。由于目标物质是在细胞内形成的（在营养限制条件下），因此在生物反应器培养中，与游离菌丝体相比，真菌菌球更受青睐。蛋白酶抑制剂生产的优化成为本研究的主要重点。研究了孢子浓度、氯化钙和普朗尼克F68的浓度对真菌生长、菌球形态以及蛋白酶抑制剂生产的影响。调整孢子浓度并添加普朗尼克F68和氯化钙的组合增加了获得所需形态的可能性。这确保了使用生物反应器系统时，菌株（IBWF 040 - 09）生产目标物质具有更好的可重复性。此外，该蛋白酶抑制剂在刃天青试验中进行了测试，结果表明对从全血细胞中分离出的外周血单核细胞没有明显的细胞毒性作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/da2df549e42a/ELSC-25-e70012-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/3b74c230fd03/ELSC-25-e70012-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/d9f78476f459/ELSC-25-e70012-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/4ffe72f31103/ELSC-25-e70012-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/9dbe48fc19b0/ELSC-25-e70012-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/e92e5ce1e5c9/ELSC-25-e70012-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/4d1dccead81d/ELSC-25-e70012-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/24420b91dda9/ELSC-25-e70012-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/da2df549e42a/ELSC-25-e70012-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/3b74c230fd03/ELSC-25-e70012-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/d9f78476f459/ELSC-25-e70012-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/4ffe72f31103/ELSC-25-e70012-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/9dbe48fc19b0/ELSC-25-e70012-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/e92e5ce1e5c9/ELSC-25-e70012-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/4d1dccead81d/ELSC-25-e70012-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/24420b91dda9/ELSC-25-e70012-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2bf/11913720/da2df549e42a/ELSC-25-e70012-g011.jpg

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本文引用的文献

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