Tamm J, Volkwein U, Kurniawan E, Becker H
J Steroid Biochem. 1987 Mar;26(3):345-8. doi: 10.1016/0022-4731(87)90099-9.
The concentrations of testosterone and its tissular metabolites were determined in testicular and epididymal tissue obtained from eleven male subjects (aged 65-85 years) after orchiectomy for prostatic cancer. The steroids were measured in different tissular compartments, i.e. testis, caput, corpus and cauda epididymis. The values (mean +/- SD; ng/g wet weight) were: Testosterone 724.0 +/- 286.0, 32.08 +/- 2.56, 41.45 +/- 1.77 and 32.24 +/- 2.14; 5 alpha-dihydrotestosterone 6.95 +/- 1.99, 9.76 +/- 2.33, 16.87 +/- 0.21 and 15.79 +/- 2.67; 5 alpha-androstane-3 alpha, 17 beta-diol 6.07 +/- 2.33, 2.17 +/- 0.24, 1.93 +/- 0.02 and 1.17 +/- 0.20; 5 alpha-androstane-3 beta, 17 beta-diol 56.66 +/- 20.97, 3.55 +/- 0.19, 2.21 +/- 0.27 and 3.34 +/- 0.32; estradiol-17 beta 5.36 +/- 3.0, 1.08 +/- 0.014, 1.44 +/- 0.038 and 1.47 +/- 0.03, respectively. Incubation of human testicular tissue with [3H]androst-5-ene-3 beta, 17 beta-diol or [3H]dihydrotestosterone showed that both androstane-diols were exclusively formed from dihydrotestosterone. Since high concentrations of 5 alpha-androstane-3 beta, 17 beta-diol are found in testicular tissue it is suggested that this steroid may be an index of seminiferous tubular function.
对11名因前列腺癌接受睾丸切除术后的男性受试者（年龄65 - 85岁）获取的睾丸和附睾组织中睾酮及其组织代谢物的浓度进行了测定。在不同的组织区域，即睾丸、附睾头、附睾体和附睾尾中对类固醇进行了测量。数值（平均值±标准差；ng/g湿重）分别为：睾酮724.0±286.0、32.08±2.56、41.45±1.77和32.24±2.14；5α - 双氢睾酮6.95±1.99、9.76±2.33、16.87±0.21和15.79±2.67；5α - 雄甾烷 - 3α,17β - 二醇6.07±2.33、2.17±0.24、1.93±0.02和1.17±0.20；5α - 雄甾烷 - 3β,17β - 二醇56.66±20.97、3.55±0.19、2.21±0.27和3.34±0.32；雌二醇 - 17β分别为5.36±3.0、1.08±0.014、1.44±0.038和1.47±0.03。用人睾丸组织与[3H] - 雄甾 - 5 - 烯 - 3β,17β - 二醇或[3H] - 双氢睾酮孵育表明，两种雄甾烷二醇均仅由双氢睾酮形成。由于在睾丸组织中发现高浓度的5α - 雄甾烷 - 3β,17β - 二醇，提示该类固醇可能是生精小管功能的一个指标。
