Yunnan Key Laboratory of Tobacco Chemistry, China tobacco yunnan industrial Co., Ltd, Kunming, P.R. China.
School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, P.R. China.
Nat Prod Res. 2023 May;37(10):1608-1616. doi: 10.1080/14786419.2022.2103554. Epub 2022 Jul 22.
Three new isochromenes, (5-methoxy-7-prenyl-1-isochromen-3-yl)methanol (), 3-(3-(hydroxymethyl)-5-methoxy-1-isochromen-7-yl)propan-1-ol (), and (5-methoxy-7-methyl-1-isochromen-3-yl)methanol (), along with three known analogues (-) were isolated from the fermentation products of a -derived endophytic fungus . Their structures were elucidated by spectroscopic methods, including extensive 1 D and 2 D NMR techniques. Compounds - and were evaluated for their anti-tobacco mosaic virus (anti-TMV) activities. The results showed that compound exhibited high anti-TMV activity with inhibition rate of 46.4%, and this rate is higher than that of positive control. Compounds , , and also showed potential anti-TMV activity with inhibition rates of 28.6, 30.5, and 26.2%, respectively. The IC of compounds - and were also tested, and showed IC values of 49.3, 22.4, 42.2, and 54.1 M, respectively.
三种新的异色烯，（5-甲氧基-7-丙烯基-1-异色烯-3-基）甲醇（），3-（3-（羟甲基）-5-甲氧基-1-异色烯-7-基）-1-丙醇（）和（5-甲氧基-7-甲基-1-异色烯-3-基）甲醇（），以及三种已知类似物（-）从 - 衍生的内生真菌的发酵产物中分离得到。通过包括广泛的 1D 和 2D NMR 技术在内的光谱方法阐明了它们的结构。测试了化合物 - 和 对烟草花叶病毒（抗 TMV）的活性。结果表明，化合物 表现出高抗 TMV 活性，抑制率为 46.4%，该抑制率高于阳性对照。化合物 ， ，和 也表现出潜在的抗 TMV 活性，抑制率分别为 28.6%，30.5%和 26.2%。还测试了化合物 - 和 的 IC，其 IC 值分别为 49.3、22.4、42.2 和 54.1μM。
