古巴老年人B1a淋巴细胞的免疫表型特征

Immunophenotypic characterization of B1a lymphocytes in Cuban older adults.

作者信息

Suárez Vianed Marsán, Hernández Imilla Casado, Ramos Elizabeth Hernández, Domínguez Gabriela Díaz, Marrero Yenisey Triana, Pérez Yaneisy Duarte, Monteagudo Arturo Chang, Pita Ana María Simón, de Los Milagros Hernández Rego Yaquima, Zamora Mary Carmen Reyes, Guerra Luis Felipe Heredia, Abraham Consuelo Milagros Macías

机构信息

Department of Immunology, Institute of Hematology and Immunology "José Manuel Ballester Santovenia", Calle 19 e/ 8 y 10, PO Box 8070, CP 10800 Vedado, Havana, Cuba.

出版信息

Exp Gerontol. 2022 Oct 1;167:111900. doi: 10.1016/j.exger.2022.111900. Epub 2022 Jul 20.

DOI:10.1016/j.exger.2022.111900

PMID:35868536

Abstract

INTRODUCTION

B1a lymphocytes are characterized by having a high capacity for self-renewal and production of natural antibodies, in a T-independent manner. There are differences in both the number and composition of mature B lymphocytes throughout life, due to the phenomenon of immunosenescence.

OBJECTIVE

To characterize the immunophenotype of B1a lymphocytes in older Cuban adults with a simplified CD19CD20CD5 panel.

METHODS

A cross-sectional study was conducted in 30 institutionalized Cuban older adults. Characterization of CD19 + CD5+, CD20 + CD5+, and CD19 + CD20+ B lymphocytes in peripheral blood was performed by flow cytometry. For the reading and analysis of the biological samples, a flow cytometer, Beckman Coulter, Gallios, was used. A Microsoft Excel database was created with the information obtained. The sample was divided by sex and age. The data were processed using the statistical program GraphPadPrism version 6.00 using the percentage values and the median for the sample description.

RESULTS

The elderly aged 80 years and over showed a decrease in the absolute count of CD19 + CD20+ B lymphocytes, as well as in the percentage and absolute count of CD19 + CD5+ B lymphocytes. On the other hand, they presented higher absolute counts of CD20 + CD5+ lymphocytes in relation to those of the <80-year-old group. Women showed higher absolute counts of CD19 + CD5+, CD20 + CD5+, and CD19 + CD20+ B lymphocyte populations.

CONCLUSIONS

The immunophenotypic characterization of B1a lymphocytes in older Cuban adults is similar to that reported by other researchers. Both age and sex influence the absolute count of these cells, being higher in women under 80 years of age.

摘要

引言

B1a淋巴细胞的特点是具有高自我更新能力，并能以非T细胞依赖的方式产生天然抗体。由于免疫衰老现象，成熟B淋巴细胞的数量和组成在一生中存在差异。

目的

用简化的CD19CD20CD5组合来描述古巴老年成年人中B1a淋巴细胞的免疫表型。

方法

对30名入住机构的古巴老年人进行了一项横断面研究。通过流式细胞术对外周血中CD19 + CD5 +、CD20 + CD5 +和CD19 + CD20 + B淋巴细胞进行表征。使用贝克曼库尔特公司的Gallios流式细胞仪对生物样本进行读取和分析。利用获得的信息创建了一个微软Excel数据库。样本按性别和年龄进行划分。使用GraphPadPrism 6.00统计程序对数据进行处理，使用百分比值和中位数来描述样本。

结果

80岁及以上的老年人CD19 + CD20 + B淋巴细胞的绝对计数以及CD19 + CD5 + B淋巴细胞的百分比和绝对计数均有所下降。另一方面，与80岁以下组相比，他们的CD20 + CD5 +淋巴细胞绝对计数更高。女性的CD19 + CD5 +、CD20 + CD5 +和CD19 + CD20 + B淋巴细胞群体的绝对计数更高。