Department of Materials Science and Engineering, Sharif University of Technology, P.O.Box: 11155-9466, Tehran, Iran.
Department of Materials Science and Engineering, Sharif University of Technology, P.O.Box: 11155-9466, Tehran, Iran.
Anal Chim Acta. 2022 Aug 8;1220:340030. doi: 10.1016/j.aca.2022.340030. Epub 2022 Jun 9.
A recyclable optical nanosensor was developed by immobilizing l-tyrosine functionalized silver nanoparticles (AgNPs) on the polyethylene terephthalate (PET) substrate for rapid determination of Pb ions. At first, the l-tyrosine functionalized AgNPs were assessed in the solution phase; the response time was lower than 15 s, and a limit of detection lower than 9 nM was obtained in the dynamic range of 1-1000 nM. For fabrication of the optical assay kit, the design of experiment (DOE) was used to optimize the immobilization efficiency of the nanoparticles on PET films by studying AgNO concentration and pH as two crucial parameters. The assay kit in optimal conditions showed a sharp localized surface plasmon resonance band suitable for sensitive determination of Pb. The fabricated sensor showed promising results for rapid determination of lead ions with the limit of detection value as low as 1 nM (S/N = 3). The sensor reproduced the obtained results even after three consecutive runs, which proved the recyclability of the optical assay kit. The recoveries of the spiked concentration in real samples were in the range of 95%-103%, which confirmed the applicability of the sensor in practical applications.
一种可回收的光学纳米传感器是通过将 l-酪氨酸功能化的银纳米粒子（AgNPs）固定在聚对苯二甲酸乙二醇酯（PET）基底上来制备的，用于快速测定 Pb 离子。首先，在溶液相中评估了 l-酪氨酸功能化的 AgNPs；在 1-1000 nM 的动态范围内，响应时间低于 15 s，检测限低于 9 nM。为了制备光学检测试剂盒，通过研究 AgNO3 浓度和 pH 这两个关键参数，使用实验设计（DOE）优化了纳米粒子在 PET 薄膜上的固定效率。在最佳条件下，该检测试剂盒显示出适合于 Pb 灵敏测定的尖锐局域表面等离子体共振带。所制备的传感器在快速测定 Pb 离子方面表现出了有前景的结果，检测限低至 1 nM（S/N = 3）。即使进行了三次连续运行，传感器也重现了获得的结果，这证明了光学检测试剂盒的可回收性。实际样品中加标浓度的回收率在 95%-103%范围内，这证实了传感器在实际应用中的适用性。
