Department of Materials Science and Engineering, Sharif University of Technology, P.O.Box: 11155-9466, Tehran, Iran.
Department of Materials Science and Engineering, Sharif University of Technology, P.O.Box: 11155-9466, Tehran, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Mar 5;268:120692. doi: 10.1016/j.saa.2021.120692. Epub 2021 Dec 2.
L-tryptophan functionalized AgNPs were successfully fabricated using a one-pot synthesis method and assessed as a colorimetric probe for rapid and accurate determination of Mg ions. The developed sensor showed a selective response towards Mg with no interference from Ca in the wide concentration range of 1-200 µM. The sensor's response was optimized in the pH range of 9-10, which can be attributed to the protonation of amine groups and their interaction with Mg ions. The stability and selectivity of the sensor were examined in different salt (NaCl) and other metal ions, respectively. The L-tryptophan-AgNPs sensor detected Mg with the limit of detection of 3 µM, which is way lower than the concentration range of magnesium in human serum (0.75-1.05 mM). The recovery values of the developed sensor were in the range of 96-102% for the determination of Mg in urine samples. The obtained performances proved the potential application of the developed sensor for clinical diagnostic of Mg ions where an accurate and rapid response is needed.
使用一锅合成法成功制备了色氨酸功能化的 AgNPs，并将其评估为一种用于快速准确测定镁离子的比色探针。所开发的传感器对镁具有选择性响应，在 1-200 μM 的宽浓度范围内不受钙的干扰。传感器的响应在 pH 值为 9-10 的范围内进行了优化，这可以归因于伯胺基团的质子化及其与镁离子的相互作用。分别在不同盐（NaCl）和其他金属离子中检查了传感器的稳定性和选择性。L-色氨酸-AgNPs 传感器检测镁的检测限为 3 μM，远低于人血清中镁的浓度范围（0.75-1.05 mM）。开发的传感器用于尿液样品中镁的测定，其回收率值在 96-102%范围内。所获得的性能证明了所开发的传感器在需要准确快速响应的镁离子临床诊断中的潜在应用。
