Department of Biotechnology, School of Biosciences and Biotechnology, Baba Ghulam Shah Badshah University, Rajouri, 185234, India.
Department of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Sci Rep. 2022 Jul 22;12(1):12548. doi: 10.1038/s41598-022-16440-8.
To investigate the impact of Glutathione (GSH) in mitigating low-temperature stress in Pusa Sheetal cv. of Solanum lycopersicum and imparting low-temperature tolerance by evaluating the different physiological responses. The plant under research was also being studied for its growth and stress tolerance. Low temperatures (LT) stress was applied to seedlings with or without GSH application 12 h before LT stress (prophylactic dose), after 12 h-LT (preemptive dose), and post 12-h recovery (curative dose). Different concentrations of GSH [0, G1 (0.5 mM), G2 (1 mM) and G3 (2 mM)] against LT stress were used. Antioxidant activities, photosynthesis, growth, and stress tolerance indices were quantified. LT stress caused an oxidative burst in S. lycopersicum seedlings of the Pusa Sheetal cv. as indicated by increased peroxidation of lipids and HO concentration. Glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities were enhanced. The best concentration was G2 (1 mM), which resulted in a rise in antioxidant activity. Moreover, a decline in lipid peroxidation and HO levels was also seen. The purpose of this study is to identify the role of GSH in reducing LT stress and to find the best dose concentration. This is the first report to assess the GSH-mediated LT stress tolerance in S. lycopersicum (Pusa Sheetal cv.). Therefore, exogenous GSH application of optimal concentration of GSH to LT stressed S. lycopersicum can be an effective approach for augmenting the plant detoxification system and promoting its growth and development.
为了研究谷胱甘肽（GSH）在减轻 Pusa Sheetal cv. 番茄幼苗低温胁迫中的作用，并通过评估不同的生理反应来赋予其低温耐受性。研究还研究了植物的生长和抗胁迫能力。在施加低温（LT）胁迫之前 12 小时（预防剂量）、施加 LT 后 12 小时（先发制人剂量）和恢复 12 小时后（治疗剂量），用或不用 GSH 处理幼苗。使用不同浓度的 GSH [0、G1（0.5mM）、G2（1mM）和 G3（2mM）] 来对抗 LT 胁迫。测定了抗氧化活性、光合作用、生长和胁迫耐受性指数。LT 胁迫导致 Pusa Sheetal cv. 番茄幼苗发生氧化爆发，表现为脂质过氧化和 HO 浓度增加。谷胱甘肽还原酶（GR）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性增强。最佳浓度为 G2（1mM），这导致抗氧化活性升高。此外，还观察到脂质过氧化和 HO 水平的下降。本研究旨在确定 GSH 在减轻 LT 胁迫中的作用，并找到最佳剂量浓度。这是首次评估 GSH 介导的 LT 胁迫耐受性在番茄（Pusa Sheetal cv.）中的报告。因此，向 LT 胁迫的番茄施用最佳浓度的外源 GSH 可能是增强植物解毒系统并促进其生长和发育的有效方法。
