Hellal F A, El-Shabrawi H M, Abd El-Hady M, Khatab I A, El-Sayed S A A, Abdelly Chedly
Plant Nutrition Dept., National Research Centre, Dokki, Cairo, Egypt.
Plant Biotechnology Dept., National Research Centre, Dokki, Cairo, Egypt.
J Genet Eng Biotechnol. 2018 Jun;16(1):203-212. doi: 10.1016/j.jgeb.2017.10.009. Epub 2017 Oct 20.
In order to investigate the effects of drought stress on germination components of barley cultivars, a laboratory experiment was conducted in a factorial randomized complete design with four replications. The controlled experiment included ten of Egyptian barley cultivars namely; (Giza 123, 124, 125, 126, 127, 129, 130, 134, 135 and 2000) as first factor. The second factor included 4 levels of drought stress inducer by applying 0, 5, 10 and 20% of polyethylene glycol-6000 (PEG) which is equivalent to four osmotic potential levels including -0.001, -0.27, -0.54 and -1.09 MPa, respectively. The results showed that, the highest reduction was related to the drought level of 20% PEG among the barley cultivars. The best cultivars in terms of germination traits were Giza 134, Giza 127, and Giza 126 this indicate their tolerance to drought stress and Giza 130, 135, 2000 cultivars was moderately tolerance and remaining is less tolerance. The protein band 27 kDa and 78 kDa showed high intensity after stress in almost all cultivars. Those two protein bands their exciting was very clear in treated barley leaf tissue. It could be related to dehydrine and oxygen evolving enhancer protein 2 (OEE2) which involved in drought stress tolerance response. Cultivars Giza 127, 130 and 134 showed highest tolerance response under drought stress. The antioxidant enzymes PAGE pattern of Peroxidase (POX), Sodium dismutase (SOD) and Ascorbate peroxidase (APX) for Barley cultivars under drought stress revealed a high activities for Giza 126, 127, 134, 136 and 2000 under -0.5 MPa osmotic stress by PEG in most of their isoforms. Based on similarity coefficient values the highest values were 1.0 with 100% similarly between tolerant cultivars Giza 130 and Giza 127. Similarly between the susceptible cultivars 125 and Giza 129 was 60%.These data confirmed by the growth parameters which we ranked as tolerant to drought stress.
为了研究干旱胁迫对大麦品种发芽成分的影响，进行了一项实验室实验，采用析因随机完全设计，重复四次。对照实验包括十个埃及大麦品种，即吉萨123、124、125、126、127、129、130、134、135和2000，作为第一个因素。第二个因素包括通过施加0%、5%、10%和20%的聚乙二醇-6000（PEG）来诱导4个干旱胁迫水平，分别相当于四个渗透势水平，即-0.001、-0.27、-0.54和-1.09兆帕。结果表明，在大麦品种中，20%PEG干旱水平下的降幅最大。就发芽性状而言，最好的品种是吉萨134、吉萨127和吉萨126，这表明它们对干旱胁迫具有耐受性，而吉萨130、135、2000品种具有中等耐受性，其余品种耐受性较差。在几乎所有品种中，27 kDa和78 kDa的蛋白条带在胁迫后显示出高强度。在处理过的大麦叶片组织中，这两条蛋白条带的激发非常明显。这可能与参与干旱胁迫耐受反应的脱水素和放氧增强蛋白2（OEE2）有关。吉萨127、130和134品种在干旱胁迫下表现出最高的耐受反应。干旱胁迫下大麦品种过氧化物酶（POX）、超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）的抗氧化酶PAGE图谱显示，在-0.5兆帕PEG渗透胁迫下，吉萨126、127、134、136和2000的大多数同工型具有高活性。基于相似系数值，耐受性品种吉萨130和吉萨127之间的最高值为1.0，相似度为100%。同样，敏感品种125和吉萨129之间的相似度为60%。这些数据通过我们列为耐旱胁迫的生长参数得到了证实。
