Kuerbannaimu K, Zhao J, Mukaidaisi A, Wang H, Zhu J, Pan W, Kasimujiang A
Department of Rehabilitation Medicine, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China.
Fifth Clinical College, Xinjiang Medical University, Urumqi 830011, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2022 Jul 20;42(7):976-987. doi: 10.12122/j.issn.1673-4254.2022.07.03.
To investigate the effect of QH06 on TNBS-induced ulcerative colitis (UC) in rats and explore the mechanisms in light of intestinal flora and intestinal immunity.
Thirty-six male Wistar rats were randomized equally into control group, UC model group, and . QH06 intervention group. The rats in the latter two groups were subjected to colonic enema with 5% TNBS/ethanol to induce UC, followed by treatment with intragastric administration of distilled water or . QH06 at the dose of 0.21 g/kg. After 14 days of treatment, the rats were examined for colon pathologies with HE staining. The mRNA and protein expression levels of IL-4, IL-10, IL-12, and IFN-γ in the colon tissues were detected using RT-qPCR and ELISA, and the expression of TLR2 protein was detected with immunohistochemistry and ELISA. Illumina Miseq platform was used for sequencing analysis of the intestinal flora of the rats with bioinformatics analysis. The correlations of the parameters of the intestinal flora with the expression levels of TLR2 and cytokines were analyzed.
The rats with TNBS- induced UC showed obvious weight loss ( < 0.01) and severe colon tissue injury with high pathological scores ( < 0.01). The protein expression levels of IFN-γ, IL-12, and TLR2 ( < 0.01) and the mRNA expression levels of IFN-γ, IL-12 and IL-10 ( < 0.05) were significantly increased in the colon tissues of the rats with UC. Illumina Miseq sequence analysis showed that in UC rats, the Shannon index ( < 0.05) ACE ( < 0.01)and Chao ( < 0.05) index for the diversity of intestinal flora both decreased with a significantly increased abundance of ( < 0.01) and a lowered abundance of ( < 0.05). Compared with the UC rats, the rats treated with . QH06 showed obvious body weight gain ( < 0.05), lessened colon injuries, lowered pathological score of the colon tissue ( < 0.05), decreased protein expressions of IFN- γ, IL- 12, and TLR2 and mRNA expressions of IFN- γ and IL-12 ( < 0.01 or 0.05), and increased protein expressions of IL- 4 ( < 0.05). The Shannon index ACE ( < 0.05) and Chao ( < 0.05) index of intestinal microflora were significantly increased, the abundance of was lowered and that of and was increased in . QH06- treated rats ( < 0.01 or 0.05). Correlation analysis showed that IFN-γ was positively correlated with the abundance of , and IFN-γ was negatively correlated with the abundance of , , ___ and __; TLR2 was negatively correlated with __, ___ and .
. QH06 can alleviate TNBS-induced colonic mucosal injury in rats, and its effect is mediated possibly by increasing the abundance of SCFA-producing bacteria such as and inhibiting abnormal immune responses mediated by TLR2.
研究QH06对三硝基苯磺酸（TNBS）诱导的大鼠溃疡性结肠炎（UC）的影响，并从肠道菌群和肠道免疫方面探讨其作用机制。
将36只雄性Wistar大鼠随机均分为对照组、UC模型组和QH06干预组。后两组大鼠采用5% TNBS/乙醇结肠灌肠诱导UC，随后分别给予蒸馏水或0.21 g/kg的QH06灌胃治疗。治疗14天后，对大鼠进行结肠病理检查（HE染色）。采用实时定量聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）检测结肠组织中白细胞介素-4（IL-4）、白细胞介素-10（IL-10）、白细胞介素-12（IL-12）和干扰素-γ（IFN-γ）的mRNA和蛋白表达水平，采用免疫组织化学和ELISA检测Toll样受体2（TLR2）蛋白的表达。利用Illumina Miseq平台对大鼠肠道菌群进行测序分析，并进行生物信息学分析。分析肠道菌群参数与TLR2及细胞因子表达水平的相关性。
TNBS诱导的UC大鼠体重明显下降（P<0.01），结肠组织损伤严重，病理评分高（P<0.01）。UC大鼠结肠组织中IFN-γ、IL-12和TLR2的蛋白表达水平（P<0.01）以及IFN-γ、IL-12和IL-10的mRNA表达水平（P<0.05）均显著升高。Illumina Miseq序列分析显示，UC大鼠肠道菌群多样性的香农指数（P<0.05）、ACE指数（P<0.01）和Chao指数（P<0.05）均降低，[某种菌]丰度显著增加（P<0.01），[另几种菌]丰度降低（P<0.05）。与UC大鼠相比，QH06治疗的大鼠体重明显增加（P<0.05），结肠损伤减轻，结肠组织病理评分降低（P<0.05），IFN-γ、IL-12和TLR2的蛋白表达以及IFN-γ和IL-12的mRNA表达降低（P<0.01或0.05），IL-4蛋白表达增加（P<0.05）。QH06治疗的大鼠肠道菌群的香农指数、ACE指数（P<0.05）和Chao指数（P<0.05）显著升高，[某种菌]丰度降低，[另几种菌]丰度增加（P<0.01或0.05）。相关性分析显示，IFN-γ与[某种菌]丰度呈正相关，IFN-γ与[另几种菌]丰度呈负相关；TLR2与[几种菌]呈负相关。
QH06可减轻TNBS诱导的大鼠结肠黏膜损伤，其作用可能是通过增加如[某种菌]等产短链脂肪酸细菌的丰度以及抑制TLR2介导的异常免疫反应来实现的。
