Faculty of Pharmacy, Department of Pharmaceutical Technology, Ege University, Izmir, Turkey.
Faculty of Pharmacy, Department of Biochemistry, Izmir Katip Çelebi University, Izmir, Turkey.
J Cosmet Dermatol. 2022 Nov;21(11):6292-6301. doi: 10.1111/jocd.15254. Epub 2022 Aug 9.
Pomegranate peel extract is known as a powerful antioxidant and due to preventing oxidation, it can reduce color change of dyed hair after washing. Liposomes are vesicular systems that include lipids and can form a film on hair fibers. Delivery system and active agent have a synergistic effect on protecting hair color and reducing dyeing frequency.
This study aimed to prepare liposomes suspension as an innovative formulation of pomegranate peel extract to reduce hair color changing.
Pomegranate peel extract-loaded liposomes were prepared with lipidic film hydration method. The characterizations of formulations (F1 and F2) were defined by several parameters. The pH, particle size, polydispersity index, zeta potential, microscopical image, loading capacity (LC), and encapsulation efficiency (EE) of formulations were determined. The antioxidant capacity of formulations and actives were tested. The effect of formulations on hair color change was shown with ex-vivo studies.
The results showed that cholesterol influenced particle size, zeta potential, and antioxidant capacity. The particle sizes of formulations were 217.71 ± 6.74 nm and 577.5 ± 1.41 nm for F1 and F2, respectively. F2 formulation had better results for zeta potential (33.8 mV) while F1 was neutral. Morphologic images confirmed vesicular structure or liposomes. The EE was found higher for F2 than F1 (F1: 57.14 and F2: 78.69). Antioxidant studies confirmed that active substance and the vesicular system had a synergistic effect on protection from oxidation. Selected formulation reduced hair color change as shown in ex-vivo tests.
Pomegranate peel extract-loaded liposomes were designed for hair color protection. It was shown with this study that prepared formulations have a good color protection on hair fibers due to antioxidant properties of pomegranate peel extract and film forming effect of liposomal formulations. According to results, prepared liposomal formulations may serve as a good alternative for reducing dyeing frequency and protecting hair fibers.
石榴皮提取物是一种强大的抗氧化剂，由于能够防止氧化，它可以减少洗发后染发的颜色变化。脂质体是包含脂质的囊泡系统，可以在头发纤维上形成一层膜。递送系统和活性剂对保护头发颜色和减少染发频率有协同作用。
本研究旨在制备石榴皮提取物脂质体悬浮液，作为一种减少头发颜色变化的创新制剂。
采用脂质体薄膜水化法制备石榴皮提取物脂质体。通过几个参数来定义配方（F1 和 F2）的特征。测定配方的 pH 值、粒径、多分散指数、Zeta 电位、显微镜图像、载药量（LC）和包封率（EE）。测试配方和活性剂的抗氧化能力。通过体外研究显示配方对头发颜色变化的影响。
结果表明，胆固醇影响粒径、Zeta 电位和抗氧化能力。配方的粒径分别为 217.71 ± 6.74nm 和 577.5 ± 1.41nm。F2 配方的 Zeta 电位（33.8 mV）更好，而 F1 为中性。形态学图像证实了囊泡结构或脂质体。F2 的 EE 高于 F1（F1：57.14，F2：78.69）。抗氧化研究证实，活性物质和囊泡系统对防止氧化具有协同作用。所选配方在体外试验中显示出减少头发颜色变化的作用。
设计了负载石榴皮提取物的脂质体用于保护头发颜色。本研究表明，由于石榴皮提取物的抗氧化特性和脂质体配方的成膜作用，所制备的配方对头发纤维具有良好的颜色保护作用。根据结果，制备的脂质体配方可能是减少染发频率和保护头发纤维的一种很好的选择。
