Manchester Institute of Biotechnology, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.
Manchester Institute of Biotechnology, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.
Curr Opin Genet Dev. 2022 Oct;76:101957. doi: 10.1016/j.gde.2022.101957. Epub 2022 Jul 20.
Saccharomyces yeasts have evolved into an important model system to study mitonuclear incompatibilities, thanks to recent advances in the field of sequencing, yeast hybridisation and multigenerational breeding. Yeast hybrids contain two homologous proteomes but retain only one type of mitochondria allowing studies on the effect of mitochondria on phenotype and gene expression. Here, we discuss the recent developments in the growing field of yeast mitogenomics spanning from the impact that this organelle has in shaping yeast fitness and genome evolution to the dissection of molecular determinants of mitonuclear incompatibilities. Applying the state-of-the-art genetic tools to a broader range of natural yeast species from different environments will help progress the field and untap the mitochondrial potential in strain development.
酿酒酵母已演变成研究线粒体-核相互作用的重要模型系统，这要归功于测序、酵母杂交和多代繁殖领域的最新进展。酵母杂种含有两个同源的蛋白质组，但只保留一种类型的线粒体，这使得研究线粒体对表型和基因表达的影响成为可能。在这里，我们讨论了酵母线粒体基因组学这一不断发展的领域的最新进展，涵盖了这个细胞器在塑造酵母适应性和基因组进化方面的影响，以及对线粒体-核不兼容分子决定因素的剖析。将最先进的遗传工具应用于来自不同环境的更广泛的天然酵母物种，将有助于推动该领域的发展，并挖掘菌株开发中的线粒体潜力。
