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藻蓝蛋白-海藻酸钠-预糊化玉米淀粉复合凝胶珠的制备及表征:载体对藻蓝蛋白动力学稳定性的影响。

Fabrication and characterization of phycocyanin-alginate-pregelatinized corn starch composite gel beads: Effects of carriers on kinetic stability of phycocyanin.

机构信息

Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran.

Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran.

出版信息

Int J Biol Macromol. 2022 Oct 1;218:665-678. doi: 10.1016/j.ijbiomac.2022.07.111. Epub 2022 Jul 21.

DOI:10.1016/j.ijbiomac.2022.07.111
PMID:35870624
Abstract

Composite gel beads using calcium alginate and different concentrations of pregelatinized corn starch (PCS) were produced to encapsulate phycocyanin (PC). Rheological properties of different sodium alginate/PCS/PC mixtures, structural and morphological properties of beads, and kinetic stability of encapsulated PC (upon heating at various time-temperature combinations) were then assessed. Rheological properties of the mixtures exhibited shear thinning behaviors. Aquagram revealed that the PC-containing beads had more water structure with weak‑hydrogen bonds. Morphological images represented less subsidence in the structures of composite gel beads, unlike PCS-free beads. Kinetic study showed that degradation rate constant values of PC encapsulated in composite gel beads (1.08-3.45 × 10, 3.38-4.43 × 10, and 5.57-15.32 × 10 s) were lower than those in PCS-free alginate gel beads (4.45 × 10, 9.20 × 10, and 18.04 × 10 s) at 40, 50, and 60 °C, respectively. This study suggests that the composite gel beads can improve PC stability.

摘要

采用海藻酸钠和不同浓度的预糊化玉米淀粉(PCS)制备复合凝胶珠,用于包埋藻蓝蛋白(PC)。然后评估了不同海藻酸钠/PCS/PC 混合物的流变性能、珠粒的结构和形态特性以及包埋 PC 的动力学稳定性(在不同时间-温度组合下加热时)。混合物的流变性能表现出剪切变稀行为。水凝胶图谱表明,含有 PC 的珠粒具有更多的水结构和较弱的氢键。形态图像显示,复合凝胶珠的结构中沉降较少,而无 PCS 的珠粒则沉降较多。动力学研究表明,复合凝胶珠中包埋的 PC 的降解速率常数值(1.08-3.45×10、3.38-4.43×10 和 5.57-15.32×10 s)低于无 PCS 的海藻酸钠凝胶珠(4.45×10、9.20×10 和 18.04×10 s)在 40、50 和 60°C 时的值。这项研究表明,复合凝胶珠可以提高 PC 的稳定性。

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