Alavi Nasireh, Golmakani Mohammad-Taghi, Hosseini Seyed Mohammad Hashem, Niakousari Mehrdad, Moosavi-Nasab Marzieh
Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran.
Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran.
Int J Biol Macromol. 2023 Jul 1;242(Pt 1):124762. doi: 10.1016/j.ijbiomac.2023.124762. Epub 2023 May 6.
Phycocyanin (PC), as a pigment-protein complex, aggregates and precipitates in acidic environments. In this context, complex formation with anionic polysaccharides is a strategy to enhance protein solubility. Besides, acidic conditions negatively affect the inherent blue color of PC, which can be prevented by encapsulation. Thereupon, in the present study, two different biopolymer-based systems, namely complexes and hydrogel beads, were prepared to increase PC solubility and its color stability under acidic conditions, respectively. Fucoidan and κ-carrageenan (KC) were separately utilized to make a complex with PC. Calcium alginate-pregelatinized corn starch (PCS) composite gel beads were used to encapsulate PC. The prepared samples were added into model systems simulating acidic conditions and then characterized during storage at 4 and 25 °C under dark conditions. Appropriate colloidal stabilities were observed for fucoidan/PC and KC/PC model systems. The color of the samples remained stable at 4 °C. As well, the bead carriers (i.e. alginate-PCS) properly protected PC against low pH conditions over time at 4 °C. Thereupon, the blue color of the beads satisfactorily remained stable at this temperature. The findings showed that complexation with fucoidan or KC and encapsulation in mixed hydrogel beads are promising routes for improving PC solubility and its color stability, respectively.
藻蓝蛋白（PC）作为一种色素 - 蛋白质复合物，在酸性环境中会聚集并沉淀。在这种情况下，与阴离子多糖形成复合物是提高蛋白质溶解度的一种策略。此外，酸性条件会对PC固有的蓝色产生负面影响，而通过包封可以防止这种情况。因此，在本研究中，分别制备了两种不同的基于生物聚合物的体系，即复合物和水凝胶珠，以分别提高PC在酸性条件下的溶解度及其颜色稳定性。岩藻依聚糖和κ-卡拉胶（KC）分别用于与PC形成复合物。海藻酸钙 - 预糊化玉米淀粉（PCS）复合凝胶珠用于包封PC。将制备的样品添加到模拟酸性条件的模型体系中，然后在4℃和25℃黑暗条件下储存期间进行表征。观察到岩藻依聚糖/PC和KC/PC模型体系具有适当的胶体稳定性。样品的颜色在4℃下保持稳定。同样，珠载体（即海藻酸 - PCS）在4℃下随着时间的推移能适当地保护PC免受低pH条件的影响。因此，在此温度下珠子的蓝色令人满意地保持稳定。研究结果表明，与岩藻依聚糖或KC形成复合物以及包封在混合水凝胶珠中分别是提高PC溶解度及其颜色稳定性的有前景的途径。
