Membrane Protective Technologies Inc, Fort Collins, Colorado, USA.
Colorado State University, Department of Biomedical Sciences, Fort Collins, Colorado, USA.
Reprod Domest Anim. 2022 Nov;57(11):1363-1374. doi: 10.1111/rda.14214. Epub 2022 Aug 1.
Commercial pooled semen from boars of maternal and terminal genetic lines was analysed over two consecutive years, as part of an external quality control program. Semen doses were prepared for two total sperm counts (2.0 × 10 /75 ml [n = 578] and 1.5 × 10 /34 ml [n = 643]). Semen doses were evaluated for motility, morphology and bacterial contamination at days 1 and 4 after collection. Additionally, temperature during transport was tracked and correlated to sperm motility. Sperm motility and morphology were significantly affected by genetic line, but not by total sperm per doses. Total motility (TM) was affected (p < .05) by season. Progressive motility (PM) was lower (p < .05) in some winter months, and higher (p < .05) in summer for maternal-line doses. Normal sperm morphology was higher (p < .05) in some months of late fall and winter, with an apparent decrease in some summer months. In general, motility was significantly higher on day 1 than on day 4, and was also affected (p < .05) by temperature, especially for terminal-line doses. Semen doses exposed to >15°C had higher (p < .05) TM, VSL (straight-line velocity) and linearity than doses exposed to <15°C. Progressive motility and straightness increased (p < .05) proportionally with temperature. At day 1, motility, average path velocity (VAP) and VSL increased (p < .05) with temperature, and was higher than the corresponding group at day 4, except for VSL. Sperm motility and kinematics at day 4 were similar between temperature groups, except for doses exposed to >20°C that had higher (p < .05) VAP and VSL than doses exposed to <12°C. Microbiology evaluation revealed that less than 8.2% (15/184) of the semen doses had bacterial growth. Sphingobacterium lactis was the most prevalent bacterium identified. In conclusion, genetic line strongly influences sperm motility, while total sperm count does not have an impact. Transient reduced temperatures during shipping do not impair sperm quality after 4 days of storage. Seasonal and temperature effects on sperm motility of pooled boar semen are not overcome by pre-production ejaculate selection and semen pooling.
商业性的公猪精液来自母系和终端遗传系，作为外部质量控制计划的一部分，连续两年进行分析。精液剂量用于两次总精子计数（2.0×10 /75 ml [n=578] 和 1.5×10 /34 ml [n=643]）。收集后第 1 天和第 4 天评估精液剂量的活力、形态和细菌污染情况。此外，还跟踪了运输过程中的温度，并将其与精子活力相关联。精子活力和形态受遗传系影响显著，但不受每剂量总精子数影响。总活力（TM）受季节影响（p<.05）。母系剂量的某些冬季月份的前向运动（PM）较低（p<.05），夏季月份的 PM 较高（p<.05）。正常精子形态在某些秋季和冬季月份较高（p<.05），而在某些夏季月份则明显下降。一般来说，第 1 天的活力明显高于第 4 天，并且还受到温度的影响（p<.05），特别是对于终端系剂量。暴露于>15°C 的精液剂量的 TM、VSL（直线速度）和直线性更高（p<.05），而暴露于<15°C 的精液剂量则更低。随着温度的升高，PM 和直线性呈比例增加（p<.05）。在第 1 天，活力、平均路径速度（VAP）和 VSL 随着温度升高而升高（p<.05），且高于第 4 天相应组，除 VSL 外。除暴露于>20°C 的剂量外，第 4 天的温度组之间的精子活力和运动学相似，暴露于<12°C 的剂量具有更高的 VAP 和 VSL（p<.05）。微生物学评估显示，不到 8.2%（15/184）的精液剂量有细菌生长。鉴定出的最常见细菌是 sphingobacterium lactis。总之，遗传系强烈影响精子活力，而总精子计数没有影响。运输过程中短暂的低温不会在储存 4 天后降低精子质量。季节性和温度对公猪精液活力的影响不能通过预生产射精选择和精液混合来克服。
