Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Setor de Suínos, Porto Alegre, Brazil.
Departamento de Zootecnia, Faculdade de Agronomia, UFRGS, Porto Alegre, Brazil.
Reprod Domest Anim. 2023 Jun;58(6):785-792. doi: 10.1111/rda.14351. Epub 2023 Apr 7.
This study evaluated the effect of sperm concentration of boar semen doses, for intrauterine artificial insemination (IUAI), on semen quality and established concentration limits for their production. Twenty ejaculates from four crossbred mature PIC boars were collected to produce 50 mL semen doses in a split sample, reaching the following sperm concentrations: ~20, 30, 60, and 100 × 10 cells/mL. Doses were produced using Androstar Plus, stored at 17°C, and evaluated until 120 h of storage. There was a linear decrease in sperm motility as the sperm concentration increased (p linear < .01). The concentration which no longer affected the total and progressive motility was 59 and 55 × 10 cells/mL, respectively (corresponding to 71% and 62%, respectively). The pH linearly decreased as the sperm concentration increased (p < .01); yet, at 72 and 120 h, the parameter dramatically reduced in boar semen doses with 60 and 100 × 10 cells/mL. The percentage of cells with intact plasma and acrosomal membranes or with high mitochondrial membrane potential was not influenced by the sperm concentration (p ≥ .15). In conclusion, sperm motility was negatively affected in highly (60 and 100 × 10 cells/mL) concentrated doses. To achieve suitable sperm motility, boar semen doses may not surpass the sperm concentration of 55 × 10 cells/mL. The effect of low-concentrated boar semen doses on sperm quality still needs to be better evaluated, mainly considering the influence of extender type and thermo-resistance conditions.
本研究评估了猪精液剂量的精子浓度对宫内人工授精（IUAI）的影响，并确定了其生产的浓度限制。从 4 头杂交 PIC 成熟公猪采集了 20 次射精，以分样方式生产 50 毫升精液剂量，达到以下精子浓度：~20、30、60 和 100×10^6 个细胞/ml。使用 Androstar Plus 生产剂量，在 17°C 下储存，并在储存 120 小时内进行评估。随着精子浓度的增加，精子活力呈线性下降（p 线性<.01）。不再影响总活力和前向运动活力的浓度分别为 59 和 55×10^6 个细胞/ml（分别对应 71%和 62%）。随着精子浓度的增加，pH 值呈线性下降（p<.01）；然而，在 72 和 120 小时时，浓度为 60 和 100×10^6 个细胞/ml 的猪精液剂量中，该参数急剧降低。具有完整质膜和顶体膜或具有高线粒体膜电位的细胞百分比不受精子浓度的影响（p≥.15）。结论是，高浓度（60 和 100×10^6 个细胞/ml）浓缩剂量会对精子活力产生负面影响。为了获得适当的精子活力，猪精液剂量不应超过 55×10^6 个细胞/ml 的浓度。还需要更好地评估低浓度猪精液剂量对精子质量的影响，特别是要考虑到扩增量类型和耐热条件的影响。
