一种新型发酵策略，可有效利用木质纤维素水解液中的木糖并生产微生物油脂。
A novel fermentation strategy for efficient xylose utilization and microbial lipid production in lignocellulosic hydrolysate.
机构信息
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing 210094, China.
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing 210094, China.
出版信息
Bioresour Technol. 2022 Oct;361:127624. doi: 10.1016/j.biortech.2022.127624. Epub 2022 Jul 21.
The sugar utilization efficiency and the tolerance of microorganism to inhibitors are essential for lipid production from lignocellulosic biomass. In this study, the sugar consumption and inhibitor tolerance characteristics of Trichosporon dermatis 32,903 were investigated. The results showed that the lipid yield on xylose was much lower than that on glucose, while these substrates exhibited comparative efficiency for cell growth. High inoculum size improved the tolerance of T. dermatis 32,903 to inhibitors. Based on these characteristics, sugar-targeted-utilization and cyclic fermentation strategy was developed. The tolerance of high inoculum size to inhibitors was utilized, glucose was targeted for lipid fermentation and xylose was targeted for cell growth. As a result, the lipid production efficiency was greatly enhanced. The lipid titer in hydrolysate of DLCA (Densifying Lignocellulosic biomass with Chemicals followed by Autoclave) pretreated rice straw was improved to as high as 38.4 g/L with lipid yield of 0.207 g/g consumed sugar.
从木质纤维素生物质生产油脂，微生物对糖的利用效率和对抑制剂的耐受性至关重要。本研究考察了地霉属 32,903 菌株的糖利用特性和抑制剂耐受性。结果表明，木糖的产油率远低于葡萄糖，但这两种底物的细胞生长效率相当。高接种量可提高地霉属 32,903 菌株对抑制剂的耐受性。基于这些特性，开发了糖靶向利用和循环发酵策略。利用高接种量对抑制剂的耐受性，靶向利用葡萄糖进行油脂发酵，靶向利用木糖进行细胞生长。结果，油脂生产效率得到了极大的提高。DLCA（用化学品致密化木质纤维素生物质，然后进行高压釜处理）预处理水稻秸秆水解液中的油脂浓度高达 38.4 g/L，消耗糖的油脂产率为 0.207 g/g。
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