Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea.
Department of Biotechnology, Graduate School, Korea University, Seoul 02841, Republic of Korea.
Bioresour Technol. 2016 Jun;209:290-6. doi: 10.1016/j.biortech.2016.02.124. Epub 2016 Mar 9.
The efficient co-fermentation of glucose and xylose is necessary for the economically feasible bioethanol production from lignocellulosic biomass. Even with xylose utilizing Saccharomyces cerevisiae, the efficiency of the lignocellulosic ethanol production remains suboptimal mainly due to the low conversion yield of xylose to ethanol. In this study, we evaluated the co-fermentation performances of SXA-R2P-E, a recently engineered isomerase-based xylose utilizing strain, in mixed sugars and in lignocellulosic hydrolysates. In a high-sugar fermentation with 70g/L of glucose and 40g/L of xylose, SXA-R2P-E produced 50g/L of ethanol with an yield of 0.43gethanol/gsugars at 72h. From dilute acid-pretreated hydrolysates of rice straw and hardwood (oak), the strain produced 18-21g/L of ethanol with among the highest yield of 0.43-0.46gethanol/gsugars ever reported. This study shows a highly promising potential of a xylose isomerase-expressing strain as an industrially relevant ethanol producer from lignocellulosic hydrolysates.
从木质纤维素生物质中经济可行地生产生物乙醇需要葡萄糖和木糖的高效共发酵。即使利用木糖利用酵母酿酒酵母，木质纤维素乙醇生产的效率仍然不理想，主要是因为木糖转化为乙醇的转化率低。在这项研究中，我们评估了最近工程化的基于异构酶的木糖利用菌株 SXA-R2P-E 在混合糖和木质纤维素水解物中的共发酵性能。在 70g/L 葡萄糖和 40g/L 木糖的高糖发酵中，SXA-R2P-E 在 72 小时内产生了 50g/L 的乙醇，得率为 0.43gethanol/gsugars。从酸预处理的水稻秸秆和硬木（橡木）水解物中，该菌株产生了 18-21g/L 的乙醇，乙醇得率为 0.43-0.46gethanol/gsugars，是迄今为止报道的最高值之一。这项研究表明，表达木糖异构酶的菌株具有很高的潜力，可以从木质纤维素水解物中生产出具有工业相关性的乙醇。
