ICAR-Directorate of Coldwater Fisheries Research, Bhimtal, 263136, Uttarakhand, India.
ICAR-Directorate of Coldwater Fisheries Research, Bhimtal, 263136, Uttarakhand, India.
Fish Shellfish Immunol. 2022 Aug;127:1033-1050. doi: 10.1016/j.fsi.2022.07.055. Epub 2022 Jul 22.
Fisheries and aquaculture industries remain essential sources of food and nutrition for millions of people worldwide. Indiscriminate use of antibiotics has led to the emergence of antimicrobial-resistant bacteria and posed a severe threat to public health. Researchers have opined that antimicrobial peptides (AMPs) can be the best possible alternative to curb the rising tide of antimicrobial resistance in aquaculture. AMPs may also help to achieve the objectives of one health approach. The natural AMPs are associated with several shortcomings, like less in vivo stability, toxicity to host cell, high cost of production and low potency in a biological system. In this review, we have provided a comprehensive outline about the strategies for designing synthetic mimics of natural AMPs with high potency. Moreover, the freely available AMP databases and the information about the molecular docking tools are enlisted. We also provided in silico template for rationally designing the AMPs from fish piscidins or other peptides. The rationally designed piscidin (rP1 and rp2) may be used to tackle microbial infections in aquaculture. Further, the protocol can be used to develop the truncated mimics of natural AMPs having more potency and protease stability.
渔业和水产养殖业仍然是全球数百万人的重要食物和营养来源。抗生素的滥用导致了抗药性细菌的出现，对公众健康构成了严重威胁。研究人员认为，抗菌肽 (AMPs) 可能是抑制水产养殖中抗药性上升的最佳替代品。AMPs 还有助于实现“同一健康”方法的目标。天然 AMPs 存在一些缺点，例如体内稳定性差、对宿主细胞的毒性、生产成本高以及在生物系统中的效力低。在这篇综述中，我们提供了关于设计具有高效力的天然 AMPs 合成模拟物的策略的全面概述。此外，还列出了可用的 AMP 数据库和有关分子对接工具的信息。我们还提供了从鱼鱼素或其他肽中合理设计 AMPs 的计算机模板。合理设计的鱼素 (rP1 和 rp2) 可用于解决水产养殖中的微生物感染问题。此外，该方案可用于开发具有更高效力和蛋白酶稳定性的天然 AMPs 的截断模拟物。
