Zhang Jing, Liu Ying, Liu Yang, Liu Wencai, Lu Fengniu, Yuan Zhiqin, Lu Chao
State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing, China.
Department of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, China.
Front Chem. 2022 Jul 8;10:928607. doi: 10.3389/fchem.2022.928607. eCollection 2022.
The exploitation of selective and sensitive dopamine (DA) sensors is essential to more deeply understand its biological function and diagnosis of related diseases. In this study, gold nanocluster-encapsulated hyperbranched polyethyleneimine (hPEI-Au NCs) has been explored as the specific and ratiometric DA nanoprobe through hPEI-assisted DA self-polymerization reactions. The Au NCs encapsulation not only provides a fluorescent internal reference but also enhances the DA self-polymerization by weakening the proton sponge effect of the hPEI layer. Rapid and sensitive DA detection is realized through the proposed hPEI-Au NC nanoprobe with a limit of detection of 10 nM. The favorable selectivity over other possible interferents including amino acids, sugars, and salts is due to the specific self-polymerization reaction. The DA analysis in urine samples with small relative standard deviations has been accomplished with an hPEI-Au NC nanoprobe.
开发选择性和灵敏的多巴胺（DA）传感器对于更深入地了解其生物学功能以及相关疾病的诊断至关重要。在本研究中，通过聚乙烯亚胺（hPEI）辅助的DA自聚合反应，探索了金纳米团簇包裹的超支化聚乙烯亚胺（hPEI-Au NCs）作为特异性和比率型DA纳米探针。Au NCs的包裹不仅提供了荧光内参，还通过削弱hPEI层的质子海绵效应增强了DA的自聚合。通过所提出的hPEI-Au NC纳米探针实现了快速灵敏的DA检测，检测限为10 nM。对包括氨基酸、糖和盐在内的其他可能干扰物具有良好的选择性，这归因于特定的自聚合反应。使用hPEI-Au NC纳米探针完成了尿液样本中DA的分析，相对标准偏差较小。
