State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China.
State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China; Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100048, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jun 5;274:121142. doi: 10.1016/j.saa.2022.121142. Epub 2022 Mar 12.
The sensitive and selective perception of dopamine (DA, a typical neurotransmitter) is important to evaluate the biological environment. In this study, a catechin-functionalized gold nanocluster (C-Au NC) nanoprobe has been explored for the ratiometric DA sensing. The detection mechanism is based on the formation of azamonardine via selective DA-catechin chemical reaction and subsequent enhanced fluorescence emission. Using Au NC emission as the internal reference, ratiometric fluorescence variation is realized, which allows sensitive DA analysis with a limit of detection of 1.0 nM (S/N = 3) and linear response concentration range from 0 to 500 nM. The characteristic chemical reaction between catechin and DA affords favorable selectivity over other amino acids, metal ions and small molecules. In addition, the practical application of the proposed nanoprobe is validated by the accurate detection of DA content in urea and cell lysate samples.
多巴胺（DA，一种典型的神经递质）的敏感和选择性感知对于评估生物环境很重要。在这项研究中，探索了一种儿茶素功能化金纳米团簇（C-Au NC）纳米探针用于比率型 DA 传感。检测机制基于 DA-儿茶素选择性化学反应形成氮杂蒙达灵和随后增强的荧光发射。利用 Au NC 发射作为内参，实现了比率荧光变化，从而可以进行灵敏的 DA 分析，检测限低至 1.0 nM（S/N = 3），线性响应浓度范围为 0 至 500 nM。儿茶素和 DA 之间的特征化学反应提供了优于其他氨基酸、金属离子和小分子的良好选择性。此外，通过对尿素和细胞裂解液样品中 DA 含量的准确检测，验证了所提出的纳米探针的实际应用。
