The Anti-Inflammatory Effect of Extract on -Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1 Signaling Axis.

() is the main pathogenic factor of gastric cancer, chronic gastritis, and other gastric diseases. It has been found that (CN) as an air-dried leaf extract has a broad-spectrum antibacterial effect. This study aims to examine the effect of CN on -infected GES-1 cells and elucidate its underlying mechanism by extracting active ingredients from air-dried leaves. GES-1 cells were cocultured with HPSS1 at MOI = 100 : 1 and treated with different concentrations of CN (100 and 200 g/ml). Results showed that CN can significantly reduce cellular LDH leakage and attenuate -induced cell apoptosis and ROS production in GSE-1 cells, so as to protect gastric epithelial cells from damage by . CN can also inhibit the secretion of inflammatory factors, such as TNF-, IL-1, IL-6, and IL-8. After CN treatment, the expression levels of active caspase-1, PYCARD, and NLRP3 were remarkably decreased in the treatment groups compared with the model group. To sum up, CN is highly protective against -induced cell damage and apoptosis; CN can depress NLRP3 inflammasome activation and ROS production via the ROS/NLRP3/caspase-1/IL-1 signaling axis to suppress -triggered inflammatory response and pyroptosis.