De Deyn P P, Marescau B, Cuykens J J, Van Gorp L, Lowenthal A, De Potter W P
Laboratory of Neurochemistry, Born-Bunge Foundation, University of Antwerp, Belgium.
Clin Chim Acta. 1987 Jul 30;167(1):81-8. doi: 10.1016/0009-8981(87)90088-x.
Twelve guanidino compounds were determined in simultaneously sampled serum and cerebrospinal fluid of eight non-dialyzed patients with renal insufficiency. Liquid cation exchange chromatography with a highly sensitive fluorescence detection method was used. In patients with serum urea levels about 10 times higher than in controls, the levels of guanidinosuccinic acid, creatinine, guanidine and methylguanidine, in serum as well as in cerebrospinal fluid, are at least 10 times higher than in control subjects. The levels of argininic acid and N-alpha-acetylarginine (in serum) and gamma-guanidinobutyric acid (in cerebrospinal fluid) are slightly increased (less than 10 X). The levels of the other guanidino compounds are close to normal values. A significant positive correlation exists between the guanidinosuccinic acid, creatinine and guanidine levels in serum and cerebrospinal fluid. The accumulation of several experimentally proven toxic guanidino compounds could contribute to the complex nervous system symptomatology and the hematological complications seen in renal insufficiency.
在8例未透析的肾功能不全患者同时采集的血清和脑脊液中测定了12种胍基化合物。采用具有高灵敏度荧光检测方法的液相阳离子交换色谱法。血清尿素水平比对照组高约10倍的患者，血清和脑脊液中的胍基琥珀酸、肌酐、胍和甲基胍水平至少比对照受试者高10倍。精氨酸和N-α-乙酰精氨酸（血清中）以及γ-胍基丁酸（脑脊液中）水平略有升高（小于10倍）。其他胍基化合物的水平接近正常值。血清和脑脊液中的胍基琥珀酸、肌酐和胍水平之间存在显著正相关。几种经实验证实有毒的胍基化合物的蓄积可能导致肾功能不全中出现的复杂神经系统症状和血液学并发症。
