Xu Yan, Yan Shaohang, Chen Can, Lu Bangbao, Zhao Ruibo
Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China.
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.
Front Cell Dev Biol. 2022 Jul 6;10:910819. doi: 10.3389/fcell.2022.910819. eCollection 2022.
Bone defects resulting from trauma or tumor are one of the most challenging problems in clinical settings. Current tissue engineering (TE) strategies for managing bone defects are insufficient, owing to without using optimal osteoconductive material and seeding cells capable of superior osteogenic potential; thus their efficacy is instable. Herein, a novel TE strategy was developed for treating bone defects. First, the decellularized bone matrix (DBM) was manufactured into powders, and these DBM powders preserved the ultrastructural and compositional properties of native trabecular bone, are non-cytotoxic and low-immunogenic, and are capable of inducing the interacted stem cells differentiating into osteogenic lineage. Then, a subtype of osteoprogenitors was isolated from mouse long bones, and its high osteogenic potential was identified . After that, we constructed a "bone-forming unit" by seeding the special subtype of osteoprogenitors onto the DBM powders. performance of the "bone-forming units" was determined by injecting into the defect site of a mouse femoral epiphysis bone defect model. The results indicated that the "bone-forming unit" was capable of enhancing bone defect healing by regulating new bone formation and remodeling. Overall, the study establishes a protocol to construct a novel "bone-forming unit," which may be an alternative strategy in future bone TE application.
由创伤或肿瘤导致的骨缺损是临床环境中最具挑战性的问题之一。由于未使用最佳的骨传导材料和具有卓越成骨潜能的种子细胞，当前用于治疗骨缺损的组织工程（TE）策略并不充分；因此其疗效不稳定。在此，我们开发了一种用于治疗骨缺损的新型TE策略。首先，将脱细胞骨基质（DBM）制成粉末，这些DBM粉末保留了天然小梁骨的超微结构和组成特性，无细胞毒性且免疫原性低，并且能够诱导相互作用的干细胞分化为成骨谱系。然后，从小鼠长骨中分离出一种骨祖细胞亚型，并鉴定了其高成骨潜能。之后，我们通过将这种特殊的骨祖细胞亚型接种到DBM粉末上构建了一个“骨形成单元”。通过将“骨形成单元”注入小鼠股骨骨骺骨缺损模型的缺损部位来测定其性能。结果表明，“骨形成单元”能够通过调节新骨形成和重塑来促进骨缺损愈合。总体而言，该研究建立了一种构建新型“骨形成单元”的方案，这可能是未来骨TE应用中的一种替代策略。
