Wang Yangyang, Qu Kuo, Lu Wenting, Zhao Peiyan, Wang Zhe, Cui Cuiyun, Liu Ye, Yang Ming, Yu Yongli, Wang Liying
Department of Molecular Biology, College of Basic Medical Sciences and Institute of Pediatrics, First Hospital of Jilin University, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
Department of Immunology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin 130021, China.
iScience. 2022 May 23;25(6):104453. doi: 10.1016/j.isci.2022.104453. eCollection 2022 Jun 17.
Neutrophils played a key role in the innate immune responses. Less is known about whether and how the neutrophils recruited in the immunization sites affecting the vaccine-induced antibody responses. In the process of evaluating the efficacy of an oil-in-water emulsion-formulated vaccine in mice, we found that neutrophils were rapidly and massively recruited to immunization sites but were barely detected in the draining lymph nodes. Interestingly, B cell-activating factor (BAFF) was abundantly expressed in the recruiting neutrophils at a very early stage. The initial neutrophil-derived BAFF firstly brought about the B cell responses in the local part, then subsequently in lymphoid organs. Activated B cells produced more BAFF through TLR9-IRF5 signaling pathway, thereby amplifying the vaccine-induced antibody responses. Suppressing BAFF in the neutrophils could weaken the B cell activation and reduce the antibody production. The data indicate that vaccines endow neutrophils with the potential to orchestrate antibody responses at immunization sites.
中性粒细胞在先天免疫反应中起关键作用。关于在免疫部位募集的中性粒细胞是否以及如何影响疫苗诱导的抗体反应，人们了解较少。在评估水包油乳剂配方疫苗对小鼠的效力过程中，我们发现中性粒细胞迅速大量募集到免疫部位，但在引流淋巴结中几乎检测不到。有趣的是，B细胞激活因子（BAFF）在极早期就在募集的中性粒细胞中大量表达。最初由中性粒细胞产生的BAFF首先引发局部的B细胞反应，随后在淋巴器官中引发反应。活化的B细胞通过TLR9-IRF5信号通路产生更多BAFF，从而放大疫苗诱导的抗体反应。抑制中性粒细胞中的BAFF会削弱B细胞活化并减少抗体产生。数据表明，疫苗赋予中性粒细胞在免疫部位协调抗体反应的潜力。
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