Colon A, Berl S, Clarke D D
Neurochem Res. 1987 May;12(5):431-8. doi: 10.1007/BF00972294.
In a previous study it was shown that the acetyl moiety can be incorporated into the protein of purified synaptosomes (1). This process was inhibited by veratridine and the inhibitory effect was counteracted by tetrodotoxin. This suggested that the flux of Na+ may be related to the acetylation process. We now report that in a sodium free medium the amount of acetylation is increased and the inhibitory effect of veratridine (veratrine) is no longer evident. The addition of Na+ leads to a decrease in acetylation in the presence of veratrine. The presence of scorpion toxin has an effect similar to that of veratrine and the two are not additive. Hence, they appear to act on a common site. Molecular sieve chromatography shows four radioactively labeled peaks, two of which are particularly affected by veratrine. We also show that [3H]acetate incorporated into synaptosomal protein can be recovered as acetyldansylhydrazide. In addition, the concentration of free and bound acetate was measured in whole brain as well as in synaptosomes.
在先前的一项研究中发现，乙酰基部分能够掺入纯化突触体的蛋白质中（1）。此过程受到藜芦碱的抑制，且该抑制作用可被河豚毒素抵消。这表明Na⁺的通量可能与乙酰化过程有关。我们现在报告，在无钠培养基中，乙酰化量增加，藜芦碱（藜芦毒素）的抑制作用不再明显。在藜芦碱存在的情况下，添加Na⁺会导致乙酰化减少。蝎毒素的存在具有与藜芦碱类似的作用，且二者无相加效应。因此，它们似乎作用于共同位点。分子筛层析显示有四个放射性标记峰，其中两个受藜芦碱影响尤为显著。我们还表明，掺入突触体蛋白中的[³H]乙酸盐可作为乙酰丹磺酰肼回收。此外，还测定了全脑以及突触体中游离和结合乙酸盐的浓度。
