通过在（L.）L. 毛状根中进行饲喂实验的生物合成研究。

Biosynthetic studies through feeding experiments in (L.) L. hairy roots.

作者信息

Lopes Adriana A, Souza Giuliana R S, de Castro França Suzelei, Lourenço Miriam V

机构信息

Biotechnology Unit, Universidade de Ribeirão Preto (UNAERP), Ribeirão Preto, SP Brazil.

出版信息

Plant Cell Tissue Organ Cult. 2022;151(1):215-219. doi: 10.1007/s11240-022-02342-0. Epub 2022 Jul 17.

DOI:10.1007/s11240-022-02342-0

PMID:35875188

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9288585/

Abstract

UNLABELLED

(L.) L. is a medicinal plant of the Asteraceae family, and several extracts and isolated compounds of (L.) L. showed a wide range of biological activities such as antimicrobial, anticancer, hepatoprotective, neuroprotective, hair growth promoting activities, and more recently against covid-19. (L.) L. hairy roots produce wedelolactone (WL), demethylwedelolactone (DWL) and 3,5-di--caffeoylquinic acid (3,5-diCQA), and there is no data in literature regarding biosynthetic pathways are involved. To verify the biosynthetic route, feeding experiments were carried out using sodium [2-C]acetate, [3-C]dl-phenylalanine, and C-labeled compounds (WL, DWL and 3,5-diCQA) were detected by ultra-high-performance liquid chromatography-quadrupole time of flight mass spectrometry (HPLC-QTOF-MS). Analysis showed that the metabolic pathways operative of coumestans (WL and DWL) are derived from acetate and shikimate pathways, while that the phenylpropanoid (3,5-diCQA) biosynthesis is exclusively from shikimate pathway.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s11240-022-02342-0.

摘要

未标记

（植物学名）是菊科的一种药用植物，该植物的几种提取物和分离出的化合物显示出广泛的生物活性，如抗菌、抗癌、保肝、神经保护、促进头发生长活性，以及最近发现的抗新冠病毒活性。（植物学名）的毛状根产生蟛蜞菊内酯（WL）、去甲基蟛蜞菊内酯（DWL）和3,5 - 二 - 咖啡酰奎宁酸（3,5 - 二CQA），但文献中没有关于其生物合成途径的相关数据。为了验证生物合成途径，进行了饲喂实验，使用[2 - C]醋酸钠、[3 - C]dl - 苯丙氨酸，并通过超高效液相色谱 - 四极杆飞行时间质谱（HPLC - QTOF - MS）检测C标记的化合物（WL、DWL和3,5 - 二CQA）。分析表明，香豆雌酚（WL和DWL）的代谢途径源自醋酸盐和莽草酸途径，而苯丙烷类（3,5 - 二CQA）的生物合成仅来自莽草酸途径。