Gadapa Swarnalatha, Battula Surendra Nath, Mor Sonia, Pushpadass Heartwin Amaladhas, Naik Laxmana Naik, Emerald Magdaline Eljeeva
College of Dairy Technology, P. V. Narsimha Rao Telangana Veterinary University, Kamareddy, Telangana 503111 India.
Dairy Chemistry Section, ICAR- National Dairy Research Institute, Southern Regional Station, Adugodi, Bengaluru, Karnataka 560030 India.
J Food Sci Technol. 2022 Sep;59(9):3669-3682. doi: 10.1007/s13197-022-05384-6. Epub 2022 Mar 15.
The main aim of this study was to deliver green tea catechins with enhanced bioavailability using niosomal system. Catechins-loaded niosomes were prepared using food grade surfactant, Tween 60 and membrane stabilizers namely, lauryl alcohol, cetyl alcohol and cholesterol by thin film hydration technique. Catechins-loaded niosomes exhibited a hydrodynamic diameter of 58.48 nm with a narrow size distribution (PDI = 0.13) and zeta potential of - 31.75 mV, suggestive for homogeneity and good stability. Niosomes entrapped about 85.82% of catechin and showed sustained release under simulated GI conditions. Morphology of niosomal vesicles were carried out using scanning electron microscopy-energy X-ray dispersion spectroscopy, transmission electron microscopy and atomic force microscopy. Fourier-transform infrared spectroscopy and High-performance liquid chromatography analysis confirmed successful encapsulation of catechins. Antioxidant activity of catechins was retained in the niosomal form. Fortification of milk with catechins loaded niosomes showed no significant changes on sensory, physicochemical properties and exhibited higher antioxidant property.
本研究的主要目的是使用囊泡系统递送生物利用度更高的绿茶儿茶素。采用食品级表面活性剂吐温60以及膜稳定剂月桂醇、十六醇和胆固醇，通过薄膜水化技术制备了负载儿茶素的囊泡。负载儿茶素的囊泡的流体动力学直径为58.48nm，粒径分布窄（多分散指数PDI = 0.13），ζ电位为-31.75mV，表明其具有均一性和良好的稳定性。囊泡包封了约85.82%的儿茶素，并在模拟胃肠道条件下呈现缓释特性。使用扫描电子显微镜-能量X射线色散光谱、透射电子显微镜和原子力显微镜对囊泡的形态进行了研究。傅里叶变换红外光谱和高效液相色谱分析证实儿茶素被成功包封。儿茶素的抗氧化活性以囊泡形式得以保留。用负载儿茶素的囊泡强化牛奶对其感官、理化性质没有显著影响，且表现出更高的抗氧化性能。
