Ameli Mojarad Melika, Ameli Mojarad Mandana, Pourmahdian Alireza
Department of Biology, Faculty of Basic Sciences, Kharrazi University, Tehran, Iran.
Department of Molecular Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Int J Mol Cell Med. 2021 Fall;10(4):258-264. doi: 10.22088/IJMCM.BUMS.10.4.258. Epub 2022 Jun 6.
Lung cancer is the second most common cancer and has high morbidity and mortality worldwide with non-small cell lung cancer (NSCLC) accounting for 85% of the cases. Over-expression of epidermal growth factor receptor (EGFR) has been clarified in different cancers, and has been shown to have a crucial role in tumor progression. In this study, we evaluated long non-coding RNA small NF90-associated RNA (snaR) expression in different EGFR-statue cell lines. Knockdown experiments were conducted to analyze snaR expression in selected cell lines. MTT and transwell assays were respectively employed to evaluate the proliferative and invasive abilities of NSCLC cells. The expression of snaR was remarkably up-regulated in SPC-A1 and A549 wild-type EGFR cell lines. Down regulation of snaR with small interfering RNA significantly inhibited cell invasion as well as proliferation of SPC-A1 and A549 cells. Our results indicate that snaR may be a potential therapeutic biomarker for NSCLC.
肺癌是第二大常见癌症，在全球范围内发病率和死亡率都很高，其中非小细胞肺癌（NSCLC）占病例的85%。表皮生长因子受体（EGFR）的过表达已在不同癌症中得到阐明，并已证明在肿瘤进展中起关键作用。在本研究中，我们评估了不同EGFR状态细胞系中长链非编码RNA小NF90相关RNA（snaR）的表达。进行了敲低实验以分析选定细胞系中的snaR表达。分别采用MTT和Transwell实验来评估NSCLC细胞的增殖和侵袭能力。snaR在SPC-A1和A549野生型EGFR细胞系中的表达显著上调。用小干扰RNA下调snaR可显著抑制SPC-A1和A549细胞的侵袭以及增殖。我们的结果表明，snaR可能是NSCLC的一种潜在治疗生物标志物。
