Song Zhiquan, Park Jun-Hee, Kim Hong-Rae, Lee Ga-Yeon, Kang Min-Jung, Kim Moo-Hwan, Pyun Jae-Chul
Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-gu, Seoul 120-749, Korea.
Electronic Convergence Division, Korea Institute of Ceramic Engineering and Technology (KICET), Jinju, 52851, Korea.
Analyst. 2022 Aug 8;147(16):3783-3794. doi: 10.1039/d2an00854h.
In this study, parylene-C films from plasma deposition as well as thermal deposition were pyrolyzed to prepare a carbon electrode for application in electrochemical immunoassays. Plasma deposition could prepare parylene-C in a faster deposition rate and more precise control over the thickness in comparison with the conventional thermal deposition. To analyze the influence of the deposition method, the crystal and electronic structures of the pyrolyzed parylene-C films obtained both deposition methods were compared using Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy. For application as a carbon electrode in immunoassays, the electrochemical properties of the pyrolyzed carbon films from two both deposition methods were analyzed, including the double layer capacitance (2.10 μF cm for plasma deposition and 2.20 μF cm for thermal deposition), the apparent electron transfer rate (approximately 1.1 × 10 cm s for both methods), and the electrochemical window (approximately -1.0 ∼ 2.1 V for both methods). Finally, the applicability of the pyrolyzed carbon electrode from parylene-C was demonstrated for the diagnosis of human hepatitis-C using various amperometric methods, such as cyclic voltammetry, chronoamperometry, square-wave voltammetry and differential pulse voltammetry.
在本研究中，对通过等离子体沉积以及热沉积制备的聚对二甲苯-C薄膜进行热解，以制备用于电化学免疫分析的碳电极。与传统热沉积相比，等离子体沉积能够以更快的沉积速率制备聚对二甲苯-C，并且对厚度的控制更精确。为了分析沉积方法的影响，使用傅里叶变换红外光谱、X射线光电子能谱和拉曼光谱对通过两种沉积方法获得的热解聚对二甲苯-C薄膜的晶体和电子结构进行了比较。对于作为免疫分析中的碳电极的应用，分析了来自两种沉积方法的热解碳膜的电化学性质，包括双层电容（等离子体沉积为2.10 μF/cm，热沉积为2.20 μF/cm）、表观电子转移速率（两种方法均约为1.1×10 cm/s）和电化学窗口（两种方法均约为-1.0 ∼ 2.1 V）。最后，使用各种安培法，如循环伏安法、计时电流法、方波伏安法和差分脉冲伏安法，证明了聚对二甲苯-C热解碳电极在人类丙型肝炎诊断中的适用性。
