Institute of Biomedical and Environmental Health Research, University of the West of Scotland.
Institute of Infection, Immunity and Inflammation, University of Glasgow.
J Vis Exp. 2022 Jul 6(185). doi: 10.3791/64159.
Osteoarthritis is the most prevalent musculoskeletal disease in people over 45, leading to an increasing economic and societal cost. Animal models are used to mimic many aspects of the disease. The present protocol describes the destabilization and cartilage scratch model (DCS) of post-traumatic osteoarthritis. Based on the widely used destabilization of the medial meniscus (DMM) model, DCS introduces three scratches on the cartilage surface. The current article highlights the steps to destabilize the knee by transecting the medial meniscotibial ligament followed by three intentional superficial scratches on the articular cartilage. The possible analysis methods by dynamic weight-bearing, microcomputed tomography, and histology are also demonstrated. While the DCS model is not recommended for studies that focus on the effect of osteoarthritis on the cartilage, it enables the study of osteoarthritis development in a shorter time window, with special focus on (1) osteophyte formation, (2) osteoarthritic and injury pain, and (3) the effect of cartilage damage in the whole joint.
骨关节炎是 45 岁以上人群中最常见的肌肉骨骼疾病，导致经济和社会成本不断增加。动物模型被用于模拟疾病的许多方面。本方案描述了创伤后骨关节炎的不稳定和软骨划痕模型（DCS）。基于广泛使用的内侧半月板不稳定（DMM）模型，DCS 在软骨表面上引入了三个划痕。本文重点介绍了通过横断内侧半月板胫韧带并在关节软骨上进行三个有意的浅表划痕来使膝关节不稳定的步骤。还展示了动态负重、微计算机断层扫描和组织学的可能分析方法。虽然 DCS 模型不推荐用于研究骨关节炎对软骨的影响，但它可以在更短的时间窗口内研究骨关节炎的发展，特别关注（1）骨赘形成，（2）骨关节炎和损伤疼痛，以及（3）整个关节中软骨损伤的影响。
