Changchun University of Chinese Medicine, School of Pharmaceutical Sciences, Changchun 130117, China.
J AOAC Int. 2022 Oct 26;105(6):1741-1754. doi: 10.1093/jaoacint/qsac079.
Rumex japonicus Houtt (R. japonicus) is used mainly to treat various skin diseases in Southeast Asia. However, there are few studies on its quality evaluation methods and antifungal activity.
To establish the quality control criteria for the effective parts from R. japonicus against psoriasis.
High-performance liquid chromatography (HPLC) was established for its fingerprint, and the similarity evaluation, cluster analysis (CA) and principal component analysis (PCA) were used to reveal the differences of those fingerprints among the tested R. japonicus. Network pharmacology analyzed the relationship between the components and psoriasis, revealing the potential targets of R. japonicus. Oxford cup anti-C. albicans experiment was used to verify the antifungal activity of R. japonicus.
HPLC was developed for the R. japonicus fingerprint by optimizing for 10 batches of quinquennial R. japonicus from different habitats; the 18 common peaks were identified with 10 characteristic peaks such as rutin, quercetin, aloe-emodin, nepodin, emodin, musizin-8-O-β-D-glucoside, chrysophanol, emodin-8-O-β-D-glucopyranoside, chrysophanol-8-O-β-D-glucopyranoside, and aloin, respectively. The network pharmacology-based analysis showed a high correlation between R. japonicus and psoriasis, revealing the potential targets of R. japonicus. The oxford cup anti-Candida albicans experiment displayed a significant activity response to emodin-8-O-β-D-glucopyranoside and the ethyl acetate fraction of R. japonicus acidic aqueous extract.
A new and optimized HPLC method was created, and the research provides an experimental basis for the development of effective drugs related to C. albicans.
The fingerprint of R. japonicus was organically combined with network pharmacology to further clarify its criteria for quality control.
酸模（ Rumex japonicus Houtt. ）在东南亚主要用于治疗各种皮肤病。然而，关于其质量评价方法和抗真菌活性的研究较少。
建立治疗银屑病的酸模有效部位的质量控制标准。
建立高效液相色谱法（HPLC）指纹图谱，并采用相似度评价、聚类分析（CA）和主成分分析（PCA）对不同产地的酸模指纹图谱进行分析。网络药理学分析了成分与银屑病之间的关系，揭示了酸模的潜在靶点。牛津杯抗白色念珠菌实验验证了酸模的抗真菌活性。
优化了 10 批不同产地的 5 年生酸模，建立了酸模指纹图谱；鉴定了 18 个共有峰，其中 10 个特征峰分别为芦丁、槲皮素、大黄素、大黄酚、大黄素、美司津-8-O-β-D-葡萄糖苷、大黄酸、大黄素-8-O-β-D-吡喃葡萄糖苷、大黄酚-8-O-β-D-吡喃葡萄糖苷和芦荟苷。基于网络药理学的分析表明，酸模与银屑病之间具有高度相关性，揭示了酸模的潜在靶点。牛津杯抗白色念珠菌实验显示，大黄素-8-O-β-D-吡喃葡萄糖苷和酸模酸性水提物的乙酸乙酯部位对白色念珠菌有明显的活性响应。
建立了一种新的优化 HPLC 方法，为开发与白色念珠菌相关的有效药物提供了实验依据。
将酸模的指纹图谱与网络药理学有机结合，进一步阐明其质量控制标准。
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