Kadir Abdul, Singh Jasdeep, Rahi Vikrant, Kumar Puneet
Department of Pharmaceutical Sciences and Technology, Maharaja Ranjit Singh Punjab Technical University, Bathinda, Punjab, 151001, India.
Department of Pharmacology, Central University of Punjab, Bathinda, Punjab, India.
Neurochem Res. 2022 Nov;47(11):3285-3297. doi: 10.1007/s11064-022-03677-y. Epub 2022 Jul 25.
Berberine due to its antioxidant properties, has been used around the globe significantly to treat several brain disorders. Also, oxidative stress is a pathological hallmark in neurodegenerative diseases like Huntington's disease (HD) and Tardive dyskinesia (TD). Berberine an alkaloid from plants has been reported to have neuroprotective potential in several animal models of neurodegenerative diseases. Hence, this study aims to evaluate the neuroprotective effect of berberine in the animal model of 3-nitropropionic acid (3-NP) induced HD and haloperidol induced tardive dyskinesia with special emphasis on its antioxidant property. The study protocol was divided into 2 phases, first phase involved the administration of 3-NP and berberine at the dose of (25, 50, and 100 mg/kg) intraperitoneally (i.p) and orally (p.o.) respectively for 21 days, and the following parameters (rotarod, narrow beam walk and photoactometer) as a measure of motor activity and striatal and cortical levels of (LPO, GSH, SOD, catalase, and nitrate) evaluated as a measure of oxidative stress were assessed for HD. Similarly in the second phase, TD was induced by using haloperidol, for 21 days and berberine at the dose of (25, 50, and 100 mg/kg) was administered, and both physical and biochemical parameters were assessed as mentioned for the HD study. The resultant data indicated that berberine attenuate 3-NP and haloperidol-induced behavioral changes and improved the antioxidant capcity in rodents. Hence berberine might be a novel therapeutic candidate to manage TD & HD.
黄连素因其抗氧化特性，在全球范围内被广泛用于治疗多种脑部疾病。此外，氧化应激是亨廷顿舞蹈病（HD）和迟发性运动障碍（TD）等神经退行性疾病的病理标志。据报道，植物中的生物碱黄连素在多种神经退行性疾病动物模型中具有神经保护潜力。因此，本研究旨在评估黄连素在3-硝基丙酸（3-NP）诱导的HD动物模型和氟哌啶醇诱导的迟发性运动障碍中的神经保护作用，特别强调其抗氧化特性。研究方案分为两个阶段，第一阶段分别以（25、50和100mg/kg）的剂量腹腔注射（i.p）和口服（p.o.）3-NP和黄连素，持续21天，并评估以下参数（转棒试验、窄梁行走试验和光感度计）作为运动活性的指标，以及评估纹状体和皮质水平的（脂质过氧化、谷胱甘肽、超氧化物歧化酶、过氧化氢酶和硝酸盐）作为氧化应激的指标，用于HD研究。同样，在第二阶段用氟哌啶醇诱导TD，持续21天，并给予（25、50和100mg/kg）剂量 的黄连素，同时评估HD研究中提到的生理和生化参数。所得数据表明，黄连素可减轻3-NP和氟哌啶醇诱导的行为变化，并提高啮齿动物的抗氧化能力。因此，黄连素可能是治疗TD和HD的新型候选药物。
