Sung Ji Yun, Joo Hong-Gu
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea.
Curr Issues Mol Biol. 2022 Jul 6;44(7):3089-3099. doi: 10.3390/cimb44070213.
Doxorubicin (DOX) and vincristine (VC) are anti-cancer drugs commonly used for lymphoma in veterinary and human medicine. However, there are several side effects caused by these drugs. In this study, the protective effects of sonicated bacterin (sBb) on dendritic cells (DCs) damaged by two anti-cancer drugs were investigated. DCs play important roles in the innate and adaptive immunity of hosts, especially activating T cells that can suppress tumor growth. The metabolic activity of DCs significantly increased after the treatment with sBb compared to that of control DCs. In addition, there was a marked change in mitochondrial integrity between DOX-treated DC and DOX + sBb-treated DCs. Flow cytometric analysis also demonstrated that sBb upregulated the expression of the surface markers of DCs, particularly CD54. In mixed lymphocyte responses, sBb significantly increased the antigen-presenting capability of DCs. In particular, sBb increased the capability of control DCs by approximately 150% and that of VC-treated DCs by 221%. These results suggest that sBb can be used as a potential immunostimulatory agent to protect DCs from anti-cancer drug-induced damage and provide fundamental information about using a combination of DCs and vincristine in immunotherapy.
阿霉素（DOX）和长春新碱（VC）是兽医和人类医学中常用于治疗淋巴瘤的抗癌药物。然而，这些药物会引发多种副作用。在本研究中，研究了超声处理的菌苗（sBb）对两种抗癌药物损伤的树突状细胞（DCs）的保护作用。DCs在宿主的固有免疫和适应性免疫中发挥重要作用，特别是激活可抑制肿瘤生长的T细胞。与对照DCs相比，用sBb处理后DCs的代谢活性显著增加。此外，DOX处理的DC和DOX + sBb处理的DCs之间线粒体完整性有明显变化。流式细胞术分析还表明，sBb上调了DCs表面标志物的表达，特别是CD54。在混合淋巴细胞反应中，sBb显著提高了DCs的抗原呈递能力。特别是，sBb使对照DCs的能力提高了约150%，使VC处理的DCs的能力提高了221%。这些结果表明，sBb可作为一种潜在的免疫刺激剂，保护DCs免受抗癌药物诱导的损伤，并为在免疫治疗中联合使用DCs和长春新碱提供基础信息。
