Rakha Shimaa I, Elmetwally Mohammed A, El-Sheikh Ali Hossam, Balboula Ahmed Zaky, Mahmoud Abdelmonem Montaser, Zaabel Samy M
Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Reproductive Biotechnology Research Laboratory, College of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Vet Sci. 2022 Jul 1;9(7):336. doi: 10.3390/vetsci9070336.
Postovulatory aging is a major problem that limits the success of many assisted reproductive technologies (ARTs). Oxidative stress is a leading cause of oocyte aging. This study investigated the effects of lycopene supplementation of in vitro maturation (IVM) medium during the aging of mouse oocytes on the oocytes' morphology and oxidative stress status. Mouse cumulus-oocyte complexes (COCs) were collected and cultured in the IVM medium either for 17 h, (freshly matured oocytes), or for 48 h, (in vitro-aged oocytes), with or without lycopene. The rate of fragmented and degenerated oocytes and the oocyte levels of hydrogen peroxide (HO), malondialdehyde (MDA), total antioxidant capacity (TAC), reduced glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) were estimated and compared. Oocytes aged with 200 nM lycopene revealed significantly less fragmentation and degeneration, lower HO and MDA levels, and higher TAC, GSH and SOD levels than those aged without lycopene. CAT levels were unchanged by lycopene treatment. Taken together, our data showed beneficial effects of lycopene during in vitro aging of mouse oocytes by reducing the oxidative stress damages that lead to their apoptosis. The present study introduces lycopene as a natural supplement to reduce the postovulatory aging-dependent abnormalities of mammalian oocytes.
排卵后老化是一个限制多种辅助生殖技术（ART）成功率的主要问题。氧化应激是卵母细胞老化的主要原因。本研究调查了在小鼠卵母细胞老化过程中，向体外成熟（IVM）培养基中添加番茄红素对卵母细胞形态和氧化应激状态的影响。收集小鼠卵丘-卵母细胞复合体（COC），并在IVM培养基中培养17小时（新鲜成熟卵母细胞）或48小时（体外老化卵母细胞），添加或不添加番茄红素。评估并比较了破碎和退化卵母细胞的比例以及卵母细胞中过氧化氢（HO）、丙二醛（MDA）、总抗氧化能力（TAC）、还原型谷胱甘肽（GSH）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的水平。与未添加番茄红素老化的卵母细胞相比，添加200 nM番茄红素老化的卵母细胞显示出明显更少的破碎和退化，更低的HO和MDA水平，以及更高的TAC、GSH和SOD水平。番茄红素处理对CAT水平没有影响。综上所述，我们的数据表明番茄红素在小鼠卵母细胞体外老化过程中具有有益作用，可减少导致其凋亡的氧化应激损伤。本研究引入番茄红素作为一种天然补充剂，以减少哺乳动物卵母细胞排卵后老化相关的异常情况。
