采用 QuEChERS 前处理和 UPLC-MS/MS 分析同时测定谷物中琥珀酸脱氢酶抑制剂类杀菌剂残留。
Simultaneous determination of succinate-dehydrogenase-inhibitor fungicide traces in cereals by QuEChERS preparation and UPLC-MS/MS analysis.
机构信息
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
出版信息
Food Chem. 2022 Dec 1;396:133708. doi: 10.1016/j.foodchem.2022.133708. Epub 2022 Jul 16.
A method for the simultaneous determination of 19 succinate dehydrogenase inhibitor (SDHI) fungicide residues in 8 kinds of cereals was established by combining UHPLC-MS/MS with the improved QuEChERS method. MgSO and octadecylsilane (C) were used as the dispersive-solid phase extraction sorbent. The proposed method had good linearity in the range of 10-100 µg/L with correlation coefficients (R > 0.99). The limit of quantification of 19 fungicides was 10 µg/L, which is the minimum addition level of the method. The fortified recoveries of 19 SDHI fungicides at three levels were ranged from 79.57 % to 126.25 %. The developed method was utilized for the analysis of 45 real cereal samples, only 5 samples were detected with SDHI fungicides. The contents of the fungicides detected in the real samples are far lower than the MRL. The results indicated that the proposed method is reliable for detecting SDHI fungicides in cereals.
建立了一种同时测定 8 种谷物中 19 种琥珀酸脱氢酶抑制剂（SDHI）杀菌剂残留的方法，该方法将超高效液相色谱-串联质谱法（UHPLC-MS/MS）与改良的 QuEChERS 法相结合。使用硫酸镁和十八烷基硅烷（C）作为分散固相萃取吸附剂。在所研究的浓度范围内，19 种杀菌剂的线性关系良好，相关系数（R>0.99）。19 种杀菌剂的定量限为 10µg/L，这是该方法的最小添加水平。19 种 SDHI 杀菌剂在三个添加水平下的加标回收率在 79.57%至 126.25%之间。该方法用于分析 45 个实际谷物样品，仅 5 个样品检测到 SDHI 杀菌剂。实际样品中检测到的杀菌剂含量远低于最大残留限量。结果表明，该方法可用于检测谷物中的 SDHI 杀菌剂。
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