Abad-Fuentes Antonio, Ceballos-Alcantarilla Eric, Mercader Josep V, Agulló Consuelo, Abad-Somovilla Antonio, Esteve-Turrillas Francesc A
Department of Biotechnology, Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Agustín Escardino 7, 46980, Paterna, Spain.
Anal Bioanal Chem. 2015 May;407(14):4207-11. doi: 10.1007/s00216-015-8608-3. Epub 2015 Mar 22.
In recent years, a second generation of succinate-dehydrogenase-inhibitor (SDHI) fungicides has been introduced into the market for effective treatment of fruit and vegetable crops, with fluxapyroxad, boscalid, fluopyram, penflufen, bixafen, penthiopyrad, and isopyrazam being some of the members of this new class of agrochemical. We herein report the development of an analytical procedure for the determination of residues of these SDHI fungicides in food samples, based on a modification of the QuEChERS extraction method followed by ultra-performance liquid chromatography coupled to tandem-mass-spectrometry determination. The proposed method reached limits of detection from 0.8 to 2.0 μg L(-1). Apple, strawberry, tomato, and spinach samples were used as model samples. Spiked samples, from 10 to 1000 μg kg(-1), were analysed by the proposed method and quantitative recoveries were obtained (from 81 to 115 % for apples, from 84 to 136 % for strawberries, from 84 to 135 % for tomatoes, and from 80 to 136 % for spinach), with precision better than 20 % in all cases. Thus, the proposed method can be used for the analysis of SDHI fungicide residues to efficiently ensure that marketed fruits and vegetables comply with the maximum residue levels established by competent authorities.
近年来，第二代琥珀酸脱氢酶抑制剂（SDHI）类杀菌剂已投放市场，用于有效防治水果和蔬菜作物，氟唑菌酰胺、啶酰菌胺、氟吡菌酰胺、戊苯吡菌胺、联苯吡菌胺、戊唑吡菌胺和异吡唑酰胺是这类新型农用化学品的部分成员。我们在此报告一种分析方法的开发，该方法基于对QuEChERS萃取方法的改进，随后采用超高效液相色谱-串联质谱法测定食品样品中这些SDHI杀菌剂的残留量。所提出的方法检测限为0.8至2.0 μg L(-1)。苹果、草莓、番茄和菠菜样品用作模型样品。对加标量为10至1000 μg kg(-1)的样品采用所提出的方法进行分析，获得了定量回收率（苹果为81%至115%，草莓为84%至136%，番茄为84%至135%，菠菜为80%至136%），所有情况下精密度均优于20%。因此，所提出的方法可用于分析SDHI杀菌剂残留量，以有效确保上市的水果和蔬菜符合主管当局规定的最大残留限量。
