Research School of Health and Welfare, School of Health and Welfare, University of Jönköping, Jönköping, Sweden.
Department of Biology and Bioinformatics, School of Bioscience, University of Skövde, Skövde, Sweden.
Sci Rep. 2022 Jul 25;12(1):12670. doi: 10.1038/s41598-022-15924-x.
Numerous studies have shown that lifestyle factors, such as regular physical activity and vitamin D intake, may remarkably improve overall health and mental wellbeing. This is especially important in older adults whose vitamin D deficiency occurs with a high prevalence. This study aimed to examine the influence of lifestyle and vitamin D on global DNA methylation patterns in an elderly cohort in Southwest of Sweden. We also sought to examine the methylation levels of specific genes involved in vitamin D's molecular and metabolic activated pathways. We performed a genome wide methylation analysis, using Illumina Infinium DNA Methylation EPIC 850kBeadChip array, on 277 healthy individuals from Southwest Sweden at the age of 70-95. The study participants also answered queries on lifestyle, vitamin intake, heart medication, and estimated health. Vitamin D intake did not in general affect methylation patterns, which is in concert with other studies. However, when comparing the group of individuals taking vitamin supplements, including vitamin D, with those not taking supplements, a difference in methylation in the solute carrier family 25 (SCL25A24) gene was found. This confirms a previous finding, where changes in expression of SLC25A24 were associated with vitamin D treatment in human monocytes. The combination of vitamin D intake and high physical activity increased methylation of genes linked to regulation of vitamin D receptor pathway, the Wnt pathway and general cancer processes. To our knowledge, this is the first study detecting epigenetic markers associated with the combined effects of vitamin D supplementation and high physical activity. These results deserve to be further investigated in an extended, interventional study cohort, where also the levels of 25(OH)D can be monitored.
大量研究表明，生活方式因素，如定期的身体活动和维生素 D 摄入，可能显著改善整体健康和心理健康。这在老年人中尤为重要，因为他们的维生素 D 缺乏症发病率很高。本研究旨在研究生活方式和维生素 D 对瑞典西南部老年人群全基因组 DNA 甲基化模式的影响。我们还试图研究参与维生素 D 分子和代谢激活途径的特定基因的甲基化水平。我们对来自瑞典西南部的 277 名健康个体（年龄在 70-95 岁之间）进行了全基因组甲基化分析，使用了 Illumina Infinium DNA Methylation EPIC 850kBeadChip 阵列。研究参与者还回答了关于生活方式、维生素摄入、心脏药物和估计健康的问题。维生素 D 摄入一般不会影响甲基化模式，这与其他研究一致。然而，当比较服用维生素补充剂（包括维生素 D）的个体与不服用补充剂的个体时，发现溶质载体家族 25（SCL25A24）基因的甲基化存在差异。这证实了之前的一项发现，即 SLC25A24 表达的变化与人类单核细胞中维生素 D 治疗有关。维生素 D 摄入和高身体活动的结合增加了与维生素 D 受体途径、Wnt 途径和一般癌症过程调节相关的基因的甲基化。据我们所知，这是首次检测到与维生素 D 补充和高身体活动联合作用相关的表观遗传标记的研究。这些结果值得在一个扩展的、干预性研究队列中进一步研究，其中也可以监测 25(OH)D 的水平。
